GJC1 antibody (AA 153-166)

Catalog No. ABIN7043207

Product Details anti-GJC1 Antibody

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Binding Specificity: AA 153-166

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Connexin-45

Specificity: Intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Connexin-45 (GJC1) Antibody is directed against an epitope of rat Connexin-45. Anti-Connexin-45 (GJC1) Antibody (ABIN7043207, ABIN7044085 and ABIN7044086)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx45 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEQSQPKPKHDGRR, corresponding to amino acid residues 153-166 of rat Connexin-45

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: BLP-CC207

Blocking Peptide: BLP-CC207

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GJC1

Target: GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))

Alternative Name: GJC1 (GJC1 Products)

Background: CX45, Gap junction γ1 protein, Gap junction α7 protein, GJA7,Gap junctions are plasma membrane domains of intercellular channels that allow direct connection between the cytoplasm of adjacent cells. They allow exchange of low molecular weight metabolites, ions and small hydrophilic molecules. These channels are formed by head-to-head docking of hexameric hemichannels called connexons, whose subunit proteins are encoded by the connexin (Cx) gene family1-3.The connexin subunit is formed of four-transmembrane spanning domains, harboring two extracellular loops, one cytoplasmic loop and cytoplasmic N- and C-terminal regions. To date, the family of connexin comprises 21 genes in the human genome4.Connexin 45 (Cx45), encoded by the GJC1 gene, is expressed in cardiomyocytes and in developing vasculature in both endothelial and smooth muscle cells4,5.In the heart, Cx45 along with Cx40 and Cx43, play a crucial role in normal electrical conduction and synchronous heart muscle contraction and important for the normal embryonic development of the heart6. Indeed Cx45 forms low conductance and voltage-sensitive gap junctions between the ventricular cardiac myocytes and the core conduction system2. Studies have showed that cardiac connexin Cx45 is upregulated in human failing hearts and has an important role in the regulation of endothelial-induced mural cell differentiation4,5.

Alternative names: Connexin-45 (GJC1), CX45, Gap junction gamma1 protein, Gap junction alpha7 protein, GJA7

Gene ID: 266706

NCBI Accession: NM_005497

UniProt: A4GG66

Pathways: Cell-Cell Junction Organization