GFAP antibody (Intracellular)

Catalog No. ABIN7043217

This anti-GFAP antibody is a Rabbit Polyclonal antibody detecting GFAP in WB, IHC and IF. Suitable for Rat.

Quick Overview for GFAP antibody (Intracellular) (ABIN7043217)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GFAP Antibody

Binding Specificity: AA 27-39, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Glial Fibrillary Acidic Protein

Specificity: Intracellular, cytoplasm

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Will not recognize human Glial fibrillary acidic protein (GFAP).

Predicted Reactivity: Mouse - 10,13 amino acid residues identical

Characteristics: Anti-GFAP Antibody (ABIN7043217, ABIN7044273 and ABIN7044274) is a highly specific antibody directed against an epitope of the rat Glial fibrillary acidic protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GFAP from rat and mouse samples. The antibody will not recognize human GFAP.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RHLGTIPRLSLSR, corresponding to amino acid residues 27-39 of rat Glial fibrillary acidic protein

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200-1:2000

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: IHC

Negative Control: (ABIN7235599)

Blocking Peptide: (ABIN7235599)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Glial fibrillary acidic protein,Glial fibrillary acidic protein (GFAP) is a key intermediate filament (IF) III protein responsible for maintaining the mechanical strength of glia cells by supporting their cytoskeleton structure. GFAP is expressed in astrocytes in the CNS, non-myelinating Schwann cells in the PNS, and enteric glial cells1.GFAP has a structural organization that is typical to class III IF proteins: it has a head, rod, and tail domains. The N-terminal head domain is important for filament formation and the C-terminal domain is important for oligomerization2.GFAP is encoded by a single gene mapped to human chromosome 17q21. To date, 10 isoforms/splice variants have been identified. GFAP is tightly regulated: both at mRNA transcription level and by phosphorylation and other post-translational modifications. A number of growth factors such as CNTF, FGF and TGF-β can induce GFAP gene transcription activation leading to increased GFAP protein levels3.Single nucleotide polymorphism (SNP) in GFAP results in the formation of Rosenthal fibers that cause Alexander Disease, hence, GFAP is a potential drug target for the treatment of this disease. A number of GFAP mutations were found in the coding and in the promoter regions of Alexander disease patients4.GFAP gene activation and protein induction appear to play a critical role in astroglia cell activation (astrogliosis) following CNS injuries and neurodegeneration. GFAP protein and its breakdown products are rapidly released into biofluids following traumatic brain and spinal cord injuries and stroke, making them strong candidate biomarkers for such neurological disorders5.

Alternative names: Glial fibrillary acidic protein, GFAP

Gene ID: 24387

NCBI Accession: NM_002055

UniProt: P47819