GLP2R antibody (Extracellular)

Catalog No. ABIN7043221

Product Details anti-GLP2R Antibody

Target: GLP2R (Glucagon-Like Peptide 2 Receptor (GLP2R))

Binding Specificity: AA 241-255, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLP2R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit  Polyclonal Antibody to Glucagon-like Peptide 2 Receptor

Specificity: 1st extracellular loop

Predicted Reactivity: Mouse,human - 13,15 amino acid residues identical

Characteristics: Anti-GLP2R (extracellular) Antibody (ABIN7043221, ABIN7044408 and ABIN7044409)) is a highly specific antibody directed against an epitpe of the rat glucagon-like peptide 2 receptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLP2R from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KRPDDESGWMSYLSE, corresponding to amino acid residues 241-255 of rat GLP2R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-GR022

Blocking Peptide: BLP-GR022

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GLP2R

Target: GLP2R (Glucagon-Like Peptide 2 Receptor (GLP2R))

Alternative Name: GLP2R (GLP2R Products)

Background: Glucagon-like peptide 2 receptor, GLP-2 receptor,Glucagon like peptide-2 (GLP-2) is a 33 amino acid peptide secreted following food consumption. It is cleaved by dipeptidyl peptidase IV thereby yielding a very short half life. GLP-2 imposes most of its effect on intestinal epithelial cells where it is also be used as a therapeutic drug for gastrointestinal injuries1-3.The many actions of GLP-2 include cellular proliferation of intestinal epithelial cells, the increase of amino acid and lipid absorption and increase of digestive enzyme activity of the epithelial brush border cells1.The GLP-2 cellular and physiological effects are primarily exerted through the glucagon-like peptide-2 receptor (GLP-2R), a member of the G-protein coupled receptor superfamily. The receptor can couple either Gs or Gi G-proteins1. However, when heterologously expressed, the receptor can stimulate the synthesis of cAMP3.Expression of GLP-2R is strongly detected in the central nervous system and the gastrointestinal tract, although its expression in this area remains controversial as some species express the receptor in different regions than others. It is also slightly detected in the lungs, cervix and vagal afferents1.

Alternative names: GLP2R, Glucagon-like peptide 2 receptor, GLP2 receptor

Gene ID: 60432

NCBI Accession: NM_004246

UniProt: Q9Z0W0

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Feeding Behaviour