Glutamate Receptor 3 antibody (Extracellular)

Catalog No. ABIN7043222

The Rabbit Polyclonal anti-Glutamate Receptor 3 antibody has been validated for WB, IHC, IF, IP, IC and LCI. It is suitable to detect Glutamate Receptor 3 in samples from Rat.

Quick Overview for Glutamate Receptor 3 antibody (Extracellular) (ABIN7043222)

Target: Glutamate Receptor 3 (GRIA3) (Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutamate Receptor 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-Glutamate Receptor 3 Antibody

Binding Specificity: AA 60-73, Extracellular

Purpose: A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 3 (GluR3)

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human,dog - identical

Characteristics: Anti-GluR3 (GluA3) (extracellular) Antibody (ABIN7043222, ABIN7044338 and ABIN7044339) is a highly specific antibody directed against an epitope of the rat ionotropic glutamate receptor 3. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, live cell imaging, and immunoprecipitation applications. It has been designed to recognize GluR3 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EKPFHLNYHVDHLD, corresponding to amino acid residues 60-73 of rat GluR3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235615)

Blocking Peptide: (ABIN7235615)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Glutamate Receptor 3

Target: Glutamate Receptor 3 (GRIA3) (Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3))

Alternative Name: GRIA3

Background: AMPA receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3,L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors).The ligand-gated ion channel family consists of 15 members that have been subdivided into three families based on their pharmacological profile: the a-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) preferring receptors, the N-methyl-D-aspartate (NMDA) preferring and the kainate preferring receptors.The AMPA receptor subfamily includes four members AMPA1 to AMPA4, also known as GluR1 to GluR4 respectively.The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3 channels, although homotetramers can also been found.AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+.1Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane potential.Synaptic strength that is defined as the level of post-synaptic depolarization can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning. Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.2The exact physiological role of the AMPA3 receptor is not clear but a role in the modulation of oscillatory networks affecting sleep and breathing has been suggested.3

Alternative names: GluR3 (GluA3), AMPA Receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3

Gene ID: 29628

NCBI Accession: NM_000828

UniProt: P19492

Pathways: PI3K-Akt Signaling, cAMP Metabolic Process, Synaptic Membrane