GRIP1 antibody (Intracellular)

Catalog No. ABIN7043227

This Rabbit Polyclonal antibody specifically detects GRIP1 in WB. It exhibits reactivity toward Rat.

Quick Overview for GRIP1 antibody (Intracellular) (ABIN7043227)

Target: GRIP1 (Glutamate Receptor Interacting Protein 1 (GRIP1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRIP1 Antibody

Binding Specificity: AA 963-977, Intracellular

Purpose: A Rabbit Polyclonal Antibody to GRIP1

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-GRIP1 Antibody (ABIN7043227, ABIN7045158 and ABIN7045159) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GRIP1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RKSVTLRKMKQEIKE, corresponding to amino acid residues 963-977 of rat GRIP1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235622)

Blocking Peptide: (ABIN7235622)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRIP1

Target: GRIP1 (Glutamate Receptor Interacting Protein 1 (GRIP1))

Alternative Name: GRIP1

Background: Glutamate receptor interacting protein 1,GRIP1 (glutamate receptor interacting protein 1) is a neuron-enriched scaffolding protein that plays an important role in regulating synaptic trafficking, synaptic strength and synaptic organization and transmission of AMPA receptor 2 and 3 (GluA2/3) 1,2. GRIP1 structure contains 7 PDZ domains responsible for protein-protein interactions. GRIP1 forms homodimers that can bind and simultaneously organize and assemble protein complexes via PDZ domains1,2.GRIP1 is abundantly expressed in glutamatergic and GABAergic synapses. In addition, GRIP1 expression can be found in muscle cells and in the epidermis1,3. Studies on GRIP1 knockout mice have revealed multi-organ developmental abnormalities. These mice die due to hemorrhage and hypovolemia in the embryonic or early post-natal period2.Mutations in GRIP1 protein resulting in a gain-of-function phenotype are linked to autism, while neuronal-specific loss-of-function mice have prosocial behavior. Other point mutations have been associated with schizophrenia and congenital kidney disease2,3.

Alternative names: GRIP1, Glutamate receptor interacting protein 1

Gene ID: 84016

NCBI Accession: NM_001178074

UniProt: P97879

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway