GRIK3 antibody (Extracellular)

Catalog No. ABIN7043239

This Rabbit Polyclonal antibody specifically detects GRIK3 in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for GRIK3 antibody (Extracellular) (ABIN7043239)

Target: GRIK3 (Glutamate Receptor, Ionotropic, Kainate 3 (GRIK3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GRIK3 Antibody

Binding Specificity: AA 46-58, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Kainate Receptor GluK3

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse - identical

Characteristics: Anti-GRIK3 (GluK3) (extracellular) Antibody is directed against an extracellular epitope of the rat kainate receptor GluK3. Anti-GRIK3 (GluK3) (extracellular) Antibody (ABIN7043239, ABIN7044378 and ABIN7044379) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus highly suited to recognize the receptor in living cells. It has been designed to recognize GluR7 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DGPNAQVMNAEEH, corresponding to amino acid residues 46-58 of rat kainate receptor GluK3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235661)

Blocking Peptide: (ABIN7235661)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRIK3

Target: GRIK3 (Glutamate Receptor, Ionotropic, Kainate 3 (GRIK3))

Alternative Name: GRIK3

Background: Glutamate receptor ionotropic kainate 3, Glutamate receptor 7, GluR7,Ionotropic glutamate receptors (iGluRs) mediate the majority of fast excitatory synaptic neurotransmission in the central nervous system. The selective assembly of iGluRs into AMPA, kainate, and N-methyl-D-aspartic acid (NMDA) receptor subtypes is regulated by their extracellular amino-terminal domains (ATDs)1.Kainate receptors (KARs) are tetramers classified into low-affinity receptor families (GluK1-GluK3) and high-affinity receptor families (GluK4-GluK5) based on their affinity for the neurotoxin kainic acid. KARs share a similar architecture with other ionotropic glutamate receptors, the subunits have a large extracellular domain composed of an amino-terminal domain (ATD) and a ligand binding domain (LBD) and an intracellular carboxy-terminal region2.KARs are expressed in neurons and glial cells throughout the CNS. GluK3 is poorly expressed, appearing in layer IV of the neocortex and dentate gyrus in the hippocampus3.KARs serve a crucial role as modulators of synaptic transmission and plasticity and their dysfunction has been linked to several disease states such as epilepsy, chronic pain and neurodegenerative diseases4. Alteration in the extracellular domain of GluK3, is in linkage disequilibrium with recurrent major depressive disorder patients5 and subjects with schizophrenia6.

Alternative names: GRIK3 (GluK3), Glutamate receptor ionotropic kainate 3, Glutamate receptor 7, GluR7

Gene ID: 298521

NCBI Accession: NM_000831

UniProt: P42264

Pathways: Synaptic Membrane