GRM8 antibody (Extracellular, N-Term)

Catalog No. ABIN7886387

The Rabbit Polyclonal anti-GRM8 antibody (ABIN7886387) specifically detects GRM8 in WB, IHC and IF. The antibody is reactive with Rat samples.

Quick Overview for GRM8 antibody (Extracellular, N-Term) (ABIN7886387)

Target: GRM8 (Glutamate Receptor, Metabotropic 8 (GRM8))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRM8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GRM8 Antibody

Binding Specificity: AA 365-377, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 8

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 12,13 amino acid residues identical

Characteristics: Anti-mGluR8 (extracellular) Antibody is directed against an extracellular epitope of rat mGluR8. Anti-mGluR8 (extracellular) Antibody (ABIN7043256 and ABIN7044363) can be used in western blot and immunohistochemistry applications. It has been designed to recognize mGluR8 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365-377 of rat mGluR8

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235709)

Blocking Peptide: (ABIN7235709)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GRM8

Target: GRM8 (Glutamate Receptor, Metabotropic 8 (GRM8))

Alternative Name: GRM8

Background:

Synonyms: Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H

Description: Metabotropic glutamate receptors (mGluRs), heptahelical transmembrane receptors coupled to G-proteins, contribute to the regulation of neuronal excitability and synaptic transmission1. Genes coding for eight mGluRs (denoted mGluR1-8) with different splice variants have been identified and classified into three groups based on sequence homology, pharmacology, and signaling pathways. Group I mGluRs (mGluR1 and mGluR5) are primarily located postsynaptically. Group II (mGluR2 and mGluR3) are present both postsynaptically and presynaptically outside the release site. Group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) couple to Gαi/o to inhibit adenylyl cyclase found in the presynaptic active zone2.The mGluR8 receptor is detected in the olfactory bulb, thalamus, pontine gray, cerebral cortex, hippocampus, cerebellum, and retina3. Electrophysiological studies suggest that mGluR8 functions as a presynaptic autoreceptor to regulate glutamate release from the lateral perforant path terminals in the mouse dentate gyrus4. It is suggested that mGluR8, like other group III mGluRs, controls glutamate release by inhibiting voltage-gated Ca2+ channels.Targeted deletion of the mGluR8 gene in mice produces subtle behavioral alterations such as reduced habituation to novelty, hyperactivity, context-dependent but not cue-dependent disruption of the freezing response in the fear conditioning test, and increased anxiety5.

Gene ID: 60590

NCBI Accession: NM_000845

UniProt: P70579

Pathways: cAMP Metabolic Process, Synaptic Membrane