GLRA3 antibody (AA 355-368)

Catalog No. ABIN7043258

Product Details anti-GLRA3 Antibody

Target: GLRA3 (GLRa3) (Glycine Receptor, alpha 3 (GLRa3))

Binding Specificity: AA 355-368

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLRA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Glycine Receptor α3

Specificity: 2nd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Glycine Receptor alpha3 Antibody is directed against an epitope of the rat glycine receptor α3. Anti-Glycine Receptor α3 Antibody (ABIN7043258 and ABIN7044396) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GlyRα3 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KNKTEAFALEKFYR, corresponding to amino acid residues 355-368 of rat GlyRalpha3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: (ABIN7235717)

Blocking Peptide: (ABIN7235717)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GLRA3

Target: GLRA3 (GLRa3) (Glycine Receptor, alpha 3 (GLRa3))

Alternative Name: GLRA3 (GLRa3 Products)

Background: Glycine receptor subunit alpha-3, GLRA3, GlyRα3,Glycine is a major neurotransmitter in the mammalian central nervous system.1 The glycine receptor is a ligand gated Cl- channel, mediating synaptic inhibition in the spinal cord, retina and regions of the brain.2The glycine receptor is a pentamer consisting of α and β subunits. To date there are four known α-subunit isoforms (α1-α4) encoded by different genes.2-4 In humans, two alternative splice variants of the glycine receptor α3 have been described: α3L and α3K, where the α3L splice variant bears an additional segment of 15 amino acids within the long cytoplasmic TM3-4 loop. These variants differ in their desensitization kinetics.3

Alternative names: Glycine Receptor alpha3, Glycine receptor subunit alpha-3, GLRA3, GlyRalpha3

Gene ID: 114516

NCBI Accession: NM_001042543

UniProt: P24524