GLYT1 antibody (Extracellular)

Catalog No. ABIN7043261

The Rabbit Polyclonal anti-GLYT1 antibody has been validated for WB, FACS and LCI. It is suitable to detect GLYT1 in samples from Rat.

Quick Overview for GLYT1 antibody (Extracellular) (ABIN7043261)

Target: GLYT1 (Glycine Transporter 1 (GLYT1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLYT1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GLYT1 Antibody

Binding Specificity: AA 202-214, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Glycine Transporter 1 (SLC6A9)

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - 12,13 amino acid residues identical

Characteristics: Anti-GlyT1 (SLC6A9) (extracellular) Antibody (ABIN7043261, ABIN7044473 and ABIN7044474) is a highly specific antibody directed against an epitope of the rat glycine transporter 1. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can thus potentially recognize GlyT1 in living cells. It has been designed to recognize GlyT1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RLYVLKLSDDIGD, corresponding to amino acid residues 202-214 of rat GlyT1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: (ABIN7235728)

Blocking Peptide: (ABIN7235728)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GLYT1

Target: GLYT1 (Glycine Transporter 1 (GLYT1))

Alternative Name: SLC6A9

Background: Na+/Cl--dependent glycine transporter 1,Apart from its obvious biochemical functions, glycine is also an important inhibitory neurotransmitter. Following depolarization, glycine is released from synaptic vesicles, binds to glycine receptors (GlyRs) on postsynaptic membranes thereby causing hyperpolarization of postsynaptic neurons due to the massive influx of Cl- ions. Glycine is then taken up from the synaptic cleft via the glycine transporters GlyT1 and GlyT21.GlyT1 and GlyT2 belong to the SLC6, Na+/Cl- dependent transporter family, of which members include transporters for GABA, serotonin, dopamine and norepinephrine2. Like all SLC6 members, GlyT1 and GlyT2 have 12 transmembrane domains and intracellular N- and C-terminals. Both can be found in different splice variants3,4. SLC6 transporters undergo post-translational modifications. For instance, GlyT1 and GlyT2 are glycosylated, which is important for their membrane trafficking5.Phosphorylation of these two transporters also takes place in a PKC-dependent manner, which may lead to down regulation of both transporters6. Pharmacologically, GlyT1 and GlyT2 can be differentiated by applying sarcosine which inhibits GlyT1 but not GlyT27.GlyT1 and GlyT2 are broadly expressed in the nervous system, GlyT1 is concentrated in glial cells, while GlyT2 is present in glycenergic neurons in the spinal cord, brainstem and cerebellum1. GlyT1 can also be detected in the pancreas, uterus, stomach, spleen, liver and retina4.GlyT1 has become a target for the treatment of schizophrenia, although a defect of the protein is not directly associated with the disorder. Inhibiting GlyT1 should lead to the increase in glutamatergic pathways, thereby decreasing psychotic effects in schizophrenic individuals. GlyT2 has been associated with hyperekplexia, a motor disorder characterized by neonatal hypertonia and startle reflex8.

Alternative names: GlyT1 (SLC6A9), Na+/Cl--dependent glycine transporter 1

Gene ID: 116509

NCBI Accession: NM_201649

UniProt: P28572