GAP43 antibody (AA 205-218)

Catalog No. ABIN7043263

This Rabbit Polyclonal antibody specifically detects GAP43 in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for GAP43 antibody (AA 205-218) (ABIN7043263)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GAP43 Antibody

Binding Specificity: AA 205-218

Purpose: A Rabbit Polyclonal Antibody to GAP43

Specificity: C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-GAP43 Antibody (ABIN7043263, ABIN7044662 and ABIN7044663) is a highly selective antibody directed against an epitope of rat Neuromodulin. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235735)

Blocking Peptide: (ABIN7235735)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43

Background: Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50,Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.

Alternative names: GAP43, Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50

Gene ID: 29423

NCBI Accession: NM_002045

UniProt: P07936