GSG1-Like antibody (Intracellular)

Catalog No. ABIN7043266

Product Details anti-GSG1-Like Antibody

Target: GSG1-Like (GSG1L)

Binding Specificity: AA 283-295, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSG1-Like antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to GSG1L

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,13 amino acid residues identical

Characteristics: Anti-GSG1L Antibody (ABIN7043266, ABIN7044390 and ABIN7044391)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GSG1L from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CRHERYPTRHQPH, corresponding to amino acid residues 283 - 295 of rat GSG1L

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GC048

Blocking Peptide: BLP-GC048

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GSG1-Like

Target: GSG1-Like (GSG1L)

Alternative Name: GSG1L (GSG1L Products)

Background: Germ cell-specific gene 1-like protein, GSG1-like protein,Germline-specific gene 1-like (GSG1L) is a tetra-spanning membrane protein which belongs to the Claudin protein superfamily. It acts as an auxiliary subunit that modulates the assembly, trafficking and gating of AMPA receptors (AMPAR)1.GSG1L has four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm, the juxtamembrane region in its C-tail extracellular Loop1 domain is critical for its suppression of AMPAR-mediated synaptic transmission2.AMPA receptors are ligand gated ion channels critical for synaptic transmission and plasticity. Their dysfunction is involved in a variety of psychiatric and neurological diseases ranging from major depressive disorder to amyotrophic lateral sclerosis. A newly explored strategy is to manipulate the activity of AMPA receptors is through compounds that affect specific AMPAR-auxiliary subunit complexes. Overexpression of GSG1L strongly suppresses AMPAR, and GSG1L knockout enhances AMPAR-mediated synaptic transmission which makes it a leading therapeutic candidate for manipulating AMPAR activity3.

Alternative names: Germ Cell-Specific Gene 1-Like Protein, GSG1-Like Protein

Gene ID: 499263

NCBI Accession: NM_144675

UniProt: D3ZK93