ChT antibody (Extracellular)

Catalog No. ABIN7043267

The Rabbit Polyclonal anti-ChT antibody has been validated for WB, IHC, IF, IC and LCI. It is suitable to detect ChT in samples from Mouse.

Quick Overview for ChT antibody (Extracellular) (ABIN7043267)

Target: ChT (High Affinity Choline Transporter (ChT))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ChT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-ChT Antibody

Binding Specificity: AA 299-311, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Choline Transporter (SLC5A7)

Specificity: 4th extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - 12,13 amino acid residues identical

Characteristics: Anti-Choline Transporter (SLC5A7) (extracellular) Antibody is directed against an epitope of mouse CHT. Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (ABIN7043267 and ABIN7044218) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CHT from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DWNQTAYGYPDPK, corresponding to amino acid residues 299-311 of mouse Choline transporter

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ChT

Target: ChT (High Affinity Choline Transporter (ChT))

Alternative Name: SLC5A7

Background: Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1,In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Following depolarization, acetylcholine is released to the synaptic cleft via synaptic vesicles and activates muscarinic and nicotinic receptors located on postsynaptic membranes1,2. Acetylcholine is then rapidly hydrolyzed into choline and acetate. Since choline is not synthesized de novo and thereby only made available through diet, choline is recycled back into the cells in order to regenerate acetylcholine. For this purpose, choline is taken up by the high affinity choline transporter (CHT)1,2.CHT has thirteen transmembrane domains, an extracellular N-terminus and an intracellular C-terminus3,4. CHT belongs to the Na+-dependent glucose transporter family (SLC5). The activity of the transporter can be confirmed by its sensitivity to hemicholimium-3 (HC-3) which inhibits the transport of choline with a Ki of 10-100 nM1. The activity of CHT reflects that of neurons1,5 i.e. increased CHT activity indicates an increase in neuronal activity. The increase in the activity of the transporter is due to the increase in the number of transporters and not due to the increase in activity per se. In addition CHT activity is also regulated by second messengers. Phosphorylation of the transporter also seems to determine the activity as it has many consensus phosphorylation sites present in the C-terminal1,2.Expression studies reveal that CHT is expressed solely in cholinergic neurons making the transporter a useful marker for detecting these types of neurons6.

Alternative names: Choline Transporter (SLC5A7), Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1

Gene ID: 63993

NCBI Accession: NM_001305005

UniProt: Q8BGY9