HRH1 antibody (AA 396-409)

Catalog No. ABIN7043268

Product Details anti-HRH1 Antibody

Target: HRH1 (Histamine Receptor H1 (HRH1))

Binding Specificity: AA 396-409

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Histamine H1 Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human,dog - 13,14 amino acid residues identical

Characteristics: Anti-Histamine H1 Receptor (HRH1) Antibody (ABIN7043268, ABIN7044505 and ABIN7044506)) is a highly specific antibody directed against an epitope of rat H1R. The antibody can be used in western blot, immunocytochemistry, indirect flow cytometry, and immunohistochemstry applications. It has been designed to recognize H1R from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat HRH1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Cited Application: IHC|IFC

Negative Control: BLP-HR001

Blocking Peptide: BLP-HR001

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HRH1

Target: HRH1 (Histamine Receptor H1 (HRH1))

Alternative Name: HRH1 (HRH1 Products)

Background: H1R, HH1R,Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.1H1 receptors are expressed in many cell types including endothelial and smooth muscle cells where they mediate vasodilatation and increased vascular permeability effects. H1R is widely distributed through the brain where it mediates sleep-wake cycles and regulates cognitive functions. The receptor is expressed in several cell populations involved in the immune response, including T-cells and B-cells, monocytes and eosinophiles.2,3H1 anti-histamine compounds have been available for almost thirty years and are among the most commonly used medication for conditions such as allergic inflammation. In addition, H1 anti-histamines are also prescribed for the treatment of insomnia and analgesia.2,3

Alternative names: Histamine H1 Receptor (HRH1), H1R, HH1R

Gene ID: 24448

NCBI Accession: NM_032369

UniProt: P31390

Pathways: Regulation of Carbohydrate Metabolic Process