HRH1 antibody (Extracellular)

Catalog No. ABIN7043269

Product Details anti-HRH1 Antibody

Target: HRH1 (Histamine Receptor H1 (HRH1))

Binding Specificity: AA 176-191, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Histamine H1 Receptor

Specificity: 2nd extracellular loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse, human - 14,16 amino acid residues identical,rat - 15

Characteristics: Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (ABIN7043269, ABIN7044512 and ABIN7044513)) is a highly specific antibody directed against an epitope of mouse H1R. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize H1R from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-HR006

Blocking Peptide: BLP-HR006

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HRH1

Target: HRH1 (Histamine Receptor H1 (HRH1))

Alternative Name: HRH1 (HRH1 Products)

Background: Histamine Receptor H1, H1R,Histamine, acts via distinct histamine H1, H2, H3, and H4 receptors, and regulates various physiological and pathological processes. Hence the activity of Histamine receptors is very crucial. They are members of G-protein coupled receptors superfamily. Thus, have seven transmembrane domains, extracellular N-terminal tail and a cytoplasmic C-terminus1.H1 receptor is excitatory and couples to Gq-type proteins, activating a signaling cascade which eventually leads to an increased cellular concentration of Ca2+ 3.H1 receptor is implicated in type I hypersensitivity allergic reactions caused by numerous allergens1. An allergic reaction occurs when allergens activate mast cells that release histamine, the released histamine binds to H1R and this causes an activation of a cascade that leads to vasodilation and vascular hyper-permeability, resulting in allergic inflammation.H1R is expressed in various tissues throughout body, including the airway, vascular smooth muscle, and brain2. H1R expressed in the brain plays an important role in the regulation of sleep-arousal cycle and memory by binding histamine where it acts as a neurotransmitter1.

Alternative names: Histamine Receptor H1, H1R

Gene ID: 15465

NCBI Accession: NM_000861

UniProt: P70174

Pathways: Regulation of Carbohydrate Metabolic Process