HOMER2 antibody (C-Term, Intracellular)

Catalog No. ABIN7887049

The Rabbit Polyclonal anti-HOMER2 antibody (ABIN7887049) specifically detects HOMER2 in WB, IHC and IF. The antibody is reactive with Rat samples.

Quick Overview for HOMER2 antibody (C-Term, Intracellular) (ABIN7887049)

Target: HOMER2 (Homer Homolog 2 (HOMER2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOMER2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-HOMER2 Antibody

Binding Specificity: AA 302-316, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Homer2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Homer2 Antibody (ABIN7043274, ABIN7045170 and ABIN7045171) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer2 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EDKVRSLKTDIEESK, corresponding to amino acid residues 302-316 of rat Homer2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235766)

Blocking Peptide: (ABIN7235766)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HOMER2

Target: HOMER2 (Homer Homolog 2 (HOMER2))

Alternative Name: HOMER2

Background:

Synonyms: Homer protein homolog 2, Cupidin, Vesl-2

Description: Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1. The Homer family includes three members, Homer1b/c, Homer2a/b, and Homer3. Homer2 protein, also called Vesl-2, is a scaffolding protein that is important for the proper organization, localization and signaling of excitatory postsynaptic receptors. Homer2 protein associates with filamentous actin (F-actin) and directly binds to drebrin, an actin-binding and remodeling protein in dendritic spines. The protein also binds to activated Cdc42, a member of the Rho small GTPase family2. The protein interacts with mGluR1, mGluR5, PI3 Kinase enhancer (PIKE), inositol 1,4,5-trisphosphate receptors (IP3), SHANK and other proteins3,4.Deletion of the Homer2 gene in mice demonstrates several abnormalities in glutamatergic signaling and in the nucleus accumbens such as reduced basal extracellular glutamate content and reduced function and total protein expression of both mGluR1 and the cystine-glutamate exchanger4.

Gene ID: 29547

NCBI Accession: NM_004839

UniProt: O88801

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling