HCRTR1 antibody (AA 256-269) (Atto 488)

Catalog No. ABIN7043286

This anti-HCRTR1 antibody is a Rabbit Polyclonal antibody detecting HCRTR1 in IHC and IF. Suitable for Rat.

Quick Overview for HCRTR1 antibody (AA 256-269) (Atto 488) (ABIN7043286)

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCRTR1 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-HCRTR1 Antibody (Atto 488)

Binding Specificity: AA 256-269

Purpose: A Rabbit Polyclonal Antibody to Orexin Receptor 1 Conjugated to the Fluorescent Dye ATTO-488

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 9,14 amino acid residues identical

Characteristics: Anti-Orexin Receptor 1 Antibody (ABIN7043285, ABIN7044883 and ABIN7044884) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX1R from rat, mouse, and human samples. \nAnti-Orexin Receptor 1-ATTO Fluor-488 Antibody (ABIN7043286) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:60

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582041)

Blocking Peptide: (ABIN7235802)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HCRTR1

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Alternative Name: HCRTR1

Background: OX1R, Hypocretin receptor type 1, HCRTR1,Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Alternative names: Orexin Receptor 1, OX1R, Hypocretin receptor type 1, HCRTR1

Gene ID: 25593

NCBI Accession: NM_001525

UniProt: P56718

Pathways: Feeding Behaviour