KCNIP1 antibody (AA 10-21)

Catalog No. ABIN7043295

Product Details anti-KCNIP1 Antibody

Target: KCNIP1 (Kv Channel Interacting Protein 1 (KCNIP1))

Binding Specificity: AA 10-21

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNIP1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to KChIP1 Subunit

Specificity: N-terminal domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-KChIP1 (KCNIP1) Antibody is directed against an epitope of rat KChIP1. Anti-KChIP1 (KCNIP1) Antibody (ABIN7043295 and ABIN7045039) can be used for western blot analysis. It has been designed to recognize KChIP1 from human mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SLQTKQRRPSKD, corresponding to amino acid residues 10-21 of rat KChIP1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: (ABIN7235837)

Blocking Peptide: (ABIN7235837)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNIP1

Target: KCNIP1 (Kv Channel Interacting Protein 1 (KCNIP1))

Alternative Name: KCNIP1 (KCNIP1 Products)

Background: KV channel-interacting protein 1, VABP, Vesicle APC-binding protein, A-type potassium channel modulatory protein 1, Potassium channel-interacting protein 1,Voltage-gated K+ (KV) channels form functional entities by the assembly of four α subunits and auxiliary subunits. Various auxiliary subunits are known to interact with KV channels thereby modulating various properties such as gating, activation and inactivation of the channels as well as influencing trafficking of the channels to the cell's plasma membrane1.KChIPs (KV channel interacting proteins) are cytoplasmic proteins which belong to the neural Ca2+ sensor (NCS) family of Ca2+ binding EF-hand proteins. To date, KChIP1-4 have been identified. All four KChIPs have a conserved C-terminal domain, which has four EF-hand-like Ca2+ binding motifs. The N-terminal region differs among the various KChIPs and attributes different properties regarding the regulation of KV channels1. KChIPs regulate different properties of KV channels such as their cell surface expression (mediated by proper trafficking of the various subunits), channel assembly and gating1-4. Specifically, KChIP1 strongly regulates the activity of the KV4 channel family1. KChIP1 has also been found to assist GABA-mediated IPSCs (inhibitory postsynaptic currents) by increasing the release of presynaptic transmitter5. Furthermore, KChIP knockout mice display an increase in anxiety-like behavior compared to their wild type counterparts5.KChIP1, 3, and 4 are mostly expressed in the brain while KChIP2 is expressed in the heart as well as in the brain4-6.

Alternative names: KChIP1 (KCNIP1), KV channel-interacting protein 1, VABP, Vesicle APC-binding protein, A-type potassium channel modulatory protein 1, Potassium channel-interacting protein 1

Gene ID: 65023

NCBI Accession: NM_001278339

UniProt: Q8R426