KCNIP2 antibody (Cytosolic)

Catalog No. ABIN7043296

Product Details anti-KCNIP2 Antibody

Target: KCNIP2 (Kv Channel Interacting Protein 2 (KCNIP2))

Binding Specificity: AA 2-15, Cytosolic

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNIP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to KChIP2 Subunit

Specificity: Cytoplasmic, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical Rat - 13,14 amino acid residues identical

Characteristics: Anti-KChIP2 Antibody (ABIN7043296, ABIN7045040 and ABIN7045041) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KChIP2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RGQGRKESLSDSRD, corresponding to amino acid residues 2-15 of human KChIP2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|ICC

Negative Control: (ABIN7235841)

Blocking Peptide: (ABIN7235841)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNIP2

Target: KCNIP2 (Kv Channel Interacting Protein 2 (KCNIP2))

Alternative Name: KCNIP2 (KCNIP2 Products)

Background: KCNIP2, KV channel-interacting protein 2, A-type potassium channel modulatory protein 2, Cardiac voltage-gated potassium channel modulatory subunit,KChIPs belong to the family of small cytosolic calcium-binding proteins and consist of four different KChIP isoforms ranging between 216 and 270 amino acids. They all have a reasonably conserved core region of 185 amino acids and a heterogeneous N-terminal. KChIPs have calcium-binding EF hands which appear to be important for modulation of KV channels but not for CaV channels1.The cardiac KChIP2 gene generates three protein isoforms of 220, 252, and 270 amino acids via alternative splicing of exons 2 and 3. It is well established that KChIP2 functionally increases peak KV4 current, slows channel inactivation and accelerates recovery from inactivation. Furthermore, KChIP2 has been reported to increase ICa,L and suggested to decrease KV1.5 cell surface expression and facilitate INa2 .All four KChIP mRNAs are found in the brain, however only KChIP2 is expressed in the heart3. KChIP2 is expressed heterogeneously across the canine and human left and right ventricular free wall with higher concentrations in epicardium compared to endocardium4.Mice deficient for KChIP2 exhibit normal cardiac structure and function but display a prolonged elevation in the ST segment on the electrocardiogram. The KChIP2-/- mice are highly susceptible to the induction of cardiac arrhythmias5.

Alternative names: KChlP2, KCNIP2, KV channel-interacting protein 2, A-type potassium channel modulatory protein 2, Cardiac voltage-gated potassium channel modulatory subunit

Gene ID: 30819

NCBI Accession: NM_014591

UniProt: Q9NS61