LRRTM1 antibody (Extracellular)

Catalog No. ABIN7043302

Product Details anti-LRRTM1 Antibody

Target: LRRTM1 (Leucine Rich Repeat Transmembrane Neuronal 1 (LRRTM1))

Binding Specificity: AA 334-346, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRRTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to LRRTM1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: rat - 12,Mouse,13 amino acid residues identical, human - 11

Characteristics: Anti-LRRTM1 (extracellular) Antibody (ABIN7043302, ABIN7044730 and ABIN7044731)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRTM1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GRYDANLQ(S)ASPE, corresponding to amino acid residues 334-346 of mouse LRRTM1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-NR141

Blocking Peptide: BLP-NR141

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LRRTM1

Target: LRRTM1 (Leucine Rich Repeat Transmembrane Neuronal 1 (LRRTM1))

Alternative Name: LRRTM1 (LRRTM1 Products)

Background: Leucine-rich repeat transmembrane neuronal protein 1,Leucine rich repeat transmembrane neuronal 1 (LRRTM1) is a synaptogenic, cell adhesion protein that contributes to the regulation of synapse density and synaptic transmission. The LRRTM family in mammals includes four postsynaptic proteins, which have been shown to interact with presynaptic neurexins and heparan sulphate proteoglycan glypican on the presynaptic side1. In human and mouse genomes, LRRTM1 is encoded by a single exon and is predominantly expressed in the nervous system. LRRTM1 is a single-membrane-spanning transmembrane protein and its structure contains ten N-terminal LRR repeats, a single pass transmembrane domain, and a cytoplasmic C-terminal tail comprising a PDZ-binding motif1.Mutations in human genes encoding the LRRTM proteins are implicated in cognitive disorders. In particular, LRRTM1 is associated to human handedness and schizophrenia1,2. Studies on Lrrtm1 knockout mice shows deficits in behavioral responses to stressful situations and novel objects, spatial memory and social discrimination deficits3. LRRTM1 mutant mice display impairment in visual behaviors, suggesting a functional role of LRRTM1 in retinothalamic convergence in vision4.

Alternative names: LRRTM1, Leucine-rich repeat transmembrane neuronal protein 1

Gene ID: 74342

NCBI Accession: NM_178839

UniProt: Q8K377

Pathways: Synaptic Membrane