LPAR1 antibody (Extracellular, N-Term)

Catalog No. ABIN7886538

This Rabbit Polyclonal antibody specifically detects LPAR1 in WB, IHC, FACS and LCI. It exhibits reactivity toward Rat.

Quick Overview for LPAR1 antibody (Extracellular, N-Term) (ABIN7886538)

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-LPAR1 Antibody

Binding Specificity: AA 36-50, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to LPA Receptor 1 (EDG2)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-LPAR1 (EDG2) (extracellular) Antibody (ABIN7043311, ABIN7044566 and ABIN7044567) is a highly specific antibody directed against an epitope of the rat LPA1 receptor. The antibody can be used in western blot analysis. It has been designed against an extracellular domain and thus is highly suitable for detecting the receptor in living cells. It has been designed to recognize LPA1 receptor from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RSGKYLATEWNTVSK, corresponding to amino acid residues 36-50 of rat LPA1 receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235898)

Blocking Peptide: (ABIN7235898)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for LPAR1

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Alternative Name: LPAR1

Background:

Synonyms: Lysophosphatidic acid receptor 1, LPA receptor 1, LPA-1, Gpcr91, Gpr26, VZG1

Description: Lysophosphatidic acid is a naturally occurring lysophospholipid that activates diverse cellular actions in various cells including proliferation and morphological changes. There are currently three known genes that encode proteins of the LPA receptor family- Edg2, Edg4 and Edg7.Lysophosphatidic acid receptor 1 (also known as Lpa1), encoded by Edg2, belongs to G-coupled protein receptor (GPCR) superfamily. All three proteins share 50-54 % identical amino-acids. Lpa1 mostly activates the Gi pathway that leads to inhibition of adenylate cyclase and thus inhibition of cAMP production. Through this pathway, Lpa1 also initiates the Ras/MAP kinase cascade which is primarily responsible for increased cell proliferation1. Interestingly, binding of lysophosphatidic acid to the G13 G-protein activates the small GTP-binding protein Rho and induces the rapid remodeling of the actin cytoskeleton2.Lpa1 has been implicated in the pathogenesis of metastatic bone cancer. Silencing of Lpa1 expression in breast and ovarian cancer cells significantly alters the progression of bone metastasis through reduction of tumor burden and inhibition of bone resorption. A specific Lpa1 antagonist exhibits specificity for tumor cell-platelet interaction without abrogating normal platelet function3.

Gene ID: 116744

NCBI Accession: NM_057159

Pathways: Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration