EDG4 antibody (Extracellular)
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- Target See all EDG4 (LPAR2) Antibodies
- EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))
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Binding Specificity
- AA 16-29, Extracellular
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This EDG4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)
- Purpose
- A Rabbit Polyclonal Antibody to LPA Receptor 2 (EDG4)
- Specificity
- Extracellular, N-terminus
- Cross-Reactivity
- Human, Mouse, Rat
- Predicted Reactivity
- Rat - identical, human - 13,14 amino acid residues identical
- Characteristics
- Anti-LPAR2 (EDG4) (extracellular) Antibody (ABIN7043312, ABIN7044568 and ABIN7044569)) is a highly specific antibody directed against an epitope of the mouse LPA2 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. It has been designed to recognize LPA2 receptor from human, rat, and mouse samples.
- Purification
- Affinity purified on immobilized antigen.
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)NSGKELSLHWRPKD, corresponding to amino acid residues 16-29 of mouse LPA2 receptor
- Isotype
- IgG
- Top Product
- Discover our top product LPAR2 Primary Antibody
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- Application Notes
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
- Comment
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Negative Control: BLP-LR032
Blocking Peptide: BLP-LR032
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))
- Alternative Name
- LPAR2 (LPAR2 Products)
- Background
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Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2,Lysophosphatidic acid (LPA) is a lipid mediator that induces various cellular responses including proliferation, migration and inhibition of apoptosis in numerous types of cells via its specific G protein-coupled receptors. At least 5 LPA G-protein coupled receptors (GPCRs) have been identified so far. LPA mediates its effects predominantly by interacting with members of the endothelial differentiation gene (EDG) subfamily of GPCRs: LPA1/EDG2, LPA2/EDG4, and LPA3/EDG71. LPA2 couples to three distinct families of heterotrimeric GTP binding proteins, including Gαq, Gαi, and Gα12/132.LPA2 spans the plasma membrane seven times and contains three extracellular loops and three intracellular loops. LPA2 is unique from the other LPA receptors as it contains two distinct protein-protein interaction domains in the carboxyl-terminal tail (aa 296-351). In the proximal region, LPA2 contains a di-leucine motif and several putative palmitoylated cysteine residues. This region is responsible for associating with zinc-finger proteins3.LPA2 mRNA is expressed in a variety of tissues including human testis, leukocytes, prostate, spleen, thymus, pancreas, and bone marrow3.LPA2-null mice do not show any obvious phenotypic aberrations5. On the other hand, LPA2 expression is increased in ovarian and thyroid cancers and inflammation, suggesting that the main function of LPA2 could become evident under pathophysiological conditions6.
Alternative names: LPAR2 (EDG4), Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2 - Gene ID
- 53978
- NCBI Accession
- NM_004720
- UniProt
- Q9JL06
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