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EDG4 antibody (Extracellular)

LPAR2 Reactivity: Mouse WB, IF (cc), LCI Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043312
  • Target See all EDG4 (LPAR2) Antibodies
    EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))
    Binding Specificity
    • 16
    • 8
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 16-29, Extracellular
    Reactivity
    • 49
    • 36
    • 30
    • 3
    Mouse
    Host
    • 65
    • 1
    Rabbit
    Clonality
    • 66
    Polyclonal
    Conjugate
    • 24
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This EDG4 antibody is un-conjugated
    Application
    • 25
    • 23
    • 21
    • 15
    • 13
    • 10
    • 9
    • 8
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)
    Purpose
    A Rabbit Polyclonal Antibody to LPA Receptor 2 (EDG4)
    Specificity
    Extracellular, N-terminus
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Rat - identical, human - 13,14 amino acid residues identical
    Characteristics
    Anti-LPAR2 (EDG4) (extracellular) Antibody (ABIN7043312, ABIN7044568 and ABIN7044569)) is a highly specific antibody directed against an epitope of the mouse LPA2 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. It has been designed to recognize LPA2 receptor from human, rat, and mouse samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)NSGKELSLHWRPKD, corresponding to amino acid residues 16-29 of mouse LPA2 receptor

    Isotype
    IgG
    Top Product
    Discover our top product LPAR2 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Comment

    Negative Control: BLP-LR032

    Blocking Peptide: BLP-LR032

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))
    Alternative Name
    LPAR2 (LPAR2 Products)
    Background
    Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2,Lysophosphatidic acid (LPA) is a lipid mediator that induces various cellular responses including proliferation, migration and inhibition of apoptosis in numerous types of cells via its specific G protein-coupled receptors. At least 5 LPA G-protein coupled receptors (GPCRs) have been identified so far. LPA mediates its effects predominantly by interacting with members of the endothelial differentiation gene (EDG) subfamily of GPCRs: LPA1/EDG2, LPA2/EDG4, and LPA3/EDG71. LPA2 couples to three distinct families of heterotrimeric GTP binding proteins, including Gαq, Gαi, and Gα12/132.LPA2 spans the plasma membrane seven times and contains three extracellular loops and three intracellular loops. LPA2 is unique from the other LPA receptors as it contains two distinct protein-protein interaction domains in the carboxyl-terminal tail (aa 296-351). In the proximal region, LPA2 contains a di-leucine motif and several putative palmitoylated cysteine residues. This region is responsible for associating with zinc-finger proteins3.LPA2 mRNA is expressed in a variety of tissues including human testis, leukocytes, prostate, spleen, thymus, pancreas, and bone marrow3.LPA2-null mice do not show any obvious phenotypic aberrations5. On the other hand, LPA2 expression is increased in ovarian and thyroid cancers and inflammation, suggesting that the main function of LPA2 could become evident under pathophysiological conditions6.

    Alternative names: LPAR2 (EDG4), Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2
    Gene ID
    53978
    NCBI Accession
    NM_004720
    UniProt
    Q9JL06
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