EDG4 antibody (Extracellular, N-Term) (FITC)

Catalog No. ABIN7043313

The FITC-conjugated Rabbit Polyclonal anti-EDG4 antibody (ABIN7043313) specifically detects EDG4 in FACS. The antibody is reactive with Mouse samples.

Quick Overview for EDG4 antibody (Extracellular, N-Term) (FITC) (ABIN7043313)

Target: EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDG4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-EDG4 Antibody (FITC)

Binding Specificity: AA 16-29, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to LPA Receptor 2 (EDG4) Conjugated to the Fluorescent Dye FITC

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-LPAR2 (EDG4) (extracellular) Antibody (ABIN7043312, ABIN7044568 and ABIN7044569) is a highly specific antibody directed against an epitope of the mouse LPA2 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. It has been designed to recognize LPA2 receptor from human, rat, and mouse samples. \nAnti-LPAR2 (EDG4) (extracellular)-FITC Antibody (ABIN7043312, ABIN7044568 and ABIN7044569)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)NSGKELSLHWRPKD, corresponding to amino acid residues 16-29 of mouse LPA2 receptor

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235902)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for EDG4

Target: EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))

Alternative Name: LPAR2

Background:

Synonyms: Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2

Description: Lysophosphatidic acid (LPA) is a lipid mediator that induces various cellular responses including proliferation, migration and inhibition of apoptosis in numerous types of cells via its specific G protein-coupled receptors. At least 5 LPA G-protein coupled receptors (GPCRs) have been identified so far. LPA mediates its effects predominantly by interacting with members of the endothelial differentiation gene (EDG) subfamily of GPCRs: LPA1/EDG2, LPA2/EDG4, and LPA3/EDG71. LPA2 couples to three distinct families of heterotrimeric GTP binding proteins, including Gαq, Gαi, and Gα12/132.LPA2 spans the plasma membrane seven times and contains three extracellular loops and three intracellular loops. LPA2 is unique from the other LPA receptors as it contains two distinct protein-protein interaction domains in the carboxyl-terminal tail (aa 296-351). In the proximal region, LPA2 contains a di-leucine motif and several putative palmitoylated cysteine residues. This region is responsible for associating with zinc-finger proteins3.LPA2 mRNA is expressed in a variety of tissues including human testis, leukocytes, prostate, spleen, thymus, pancreas, and bone marrow3.LPA2-null mice do not show any obvious phenotypic aberrations5. On the other hand, LPA2 expression is increased in ovarian and thyroid cancers and inflammation, suggesting that the main function of LPA2 could become evident under pathophysiological conditions6.

Gene ID: 53978

NCBI Accession: NM_004720

UniProt: Q9JL06