LPAR4 antibody (Extracellular)

Catalog No. ABIN7043315

Product Details anti-LPAR4 Antibody

Target: LPAR4 (Lysophosphatidic Acid Receptor 4 (LPAR4))

Binding Specificity: AA 3-16, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to LPA Receptor 4 (P2Y9)

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - identical,human - 13,14 amino acid residues identical

Characteristics: Anti-LPAR4 (P2Y9) (extracellular) Antibody is directed against an epitope of the mouse LPA4 receptor. Anti-LPAR4 (P2Y9) (extracellular) Antibody (ABIN7043315, ABIN7044572 and ABIN7044573) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA4 receptor from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRRFIDFQFQDLNS, corresponding to amino acid residues 3-16 of mouse LPA4 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: (ABIN7235905)

Blocking Peptide: (ABIN7235905)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LPAR4

Target: LPAR4 (Lysophosphatidic Acid Receptor 4 (LPAR4))

Alternative Name: LPAR4 (LPAR4 Products)

Background: Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, P2Y purinoceptor 9, Purinergic receptor 9, P2RY9, GPR23,Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptor have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family. LPA4, a member of the purinergic receptor family, and the recently identified LPA5 are structurally distant from the canonical Edg LPA1-3 receptors. LPA4 and LPA5 are linked to Gq, G12/13 and Gs but not Gi, while LPA1-3 all couple to Gi in addition to Gq and G12/132.LPA4 (P2Y9/GPR23) was cloned as an orphan GPCR and is closely related to the purinergic P2Y receptors3. Like all GPCRs it has seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.LPA4 is weakly expressed, there are high levels only in ovary2.LPA4-deficient mice display abnormalities in the blood vascular systems, which caused lethality of embryos and neonates5.

Alternative names: LPAR4 (P2Y9), Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, P2Y purinoceptor 9, Purinergic receptor 9, P2RY9, GPR23

Gene ID: 78134

NCBI Accession: NM_005296

UniProt: Q8BLG2