LPAR5 antibody (3rd Extracellular Loop)

Catalog No. ABIN7886542

The Rabbit Polyclonal anti-LPAR5 antibody (ABIN7886542) specifically detects LPAR5 in WB. The antibody is reactive with Human samples.

Quick Overview for LPAR5 antibody (3rd Extracellular Loop) (ABIN7886542)

Target: LPAR5 (Lysophosphatidic Acid Receptor 5 (LPAR5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LPAR5 Antibody

Binding Specificity: 3rd Extracellular Loop, AA 261-276

Purpose: A Rabbit Polyclonal Antibody to LPA Receptor 5

Cross-Reactivity (Details): Will not recognize rat or mouse LPA5 receptor.

Predicted Reactivity: Mouse,Rat - not recommended for use with mouse,rat samples

Characteristics: Anti-Human LPAR5 (extracellular) Antibody is directed against an epitope of the human LPA5 receptor. Anti-Human LPAR5 (extracellular) Antibody (ABIN7043316, ABIN7044574 and ABIN7044575) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA5 receptor from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RSKLVAASVPARDRVR, corresponding to amino acid residues 261-276 of human LPA5 receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for LPAR5

Target: LPAR5 (Lysophosphatidic Acid Receptor 5 (LPAR5))

Alternative Name: LPAR5

Background:

Synonyms: Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93

Description: LPA (lysophosphatidic acid) mediates cellular differentiation, growth, survival and motility through two subgroups of G-protein coupled receptors (GPCRs): The Edg (endothelial differentiation gene) family - LPA1, LPA2, LPA3 and the non-Edg family - LPA4, LPA5, LPA61.Like all GPCRs, LPA receptors have seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail1.Alkyl-LPA, commonly found in platelets activates LPA5, thus explaining the high expression of the receptor in platelets2. LPA is expressed in astrocytes, and sensory and motor neurons in the spinal cord. It is also detected in spleen, heart, placenta, lliver and colon3.LPA5 activation leads to cAMP production, however, it does so in a Gs independent manner4,5. The receptor couples Gq, thus leading to Ca2+ mobilization4.Activation of LPA5 was also shown to initiate neuropathic pain in mouse models. LPA5 knockout mice do not develop neuropathic pain6.

Gene ID: 57121

NCBI Accession: NM_020400

UniProt: Q9H1C0