MAGT1 antibody (Extracellular)

Catalog No. ABIN7043318

Product Details anti-MAGT1 Antibody

Target: MAGT1 (Magnesium Transporter 1 (MAGT1))

Binding Specificity: AA 28-40, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAGT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal antibody to MAGT1 (extracellular) magnesium transporter.

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): The immunizing peptide bears some homology (7 out of 13 amino acid residues) with the related protein TUSC3 (Accession Q13454).

Predicted Reactivity: Mouse - identical Rat 11 out of 13 amino acid residues identical

Characteristics: Anti-MAGT1 (extracellular) Antibody (ABIN7043318, ABIN7044863 and ABIN7044864) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize MAGT1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SAQRKKEMVLSEK, corresponding to amino acid residues 28-40 of human MAGT1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235907)

Blocking Peptide: (ABIN7235907)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MAGT1

Target: MAGT1 (Magnesium Transporter 1 (MAGT1))

Alternative Name: MAGT1 (MAGT1 Products)

Background: Magnesium Transporter 1, Oligosaccharyl Transferase Subunit MAGT1, Implantation-Associated Protein, SLC58A1,Magnesium Transporter 1 (MAGT1) is a 335 amino acid peptide that is a part of the MAGT group of magnesium transporters. Magnesium (Mg2+) plays an essential cellular role, as a cofactor for ATP, nucleic acids, and numerous enzymes. It has been reported that it serves as a second messenger in intracellular signaling, and hence magnesium levels maintenance has a vast effect on cellular steady state 1. In addition to its Mg2+ transporter activity, MAGT1 was also found to be a subunit of the oligosaccharyltransferase (OST) complex, through which it is responsible for protein glycosylation 2,3.MAGT1 was shown to be related to the development of XMEN (X-linked immunodeficiency with magnesium defect, Epstein Barr Virus infection, and neoplasia) syndrome, for which Mg2+ supplementation has been shown to be beneficial. It is also related to atypical ATRX syndrome, intellectual disability and skin abnormalities.Understanding the molecular basis of the involvement of magnesium and its channels in human pathogenesis can lead to the development of new medications for many diseases.

Alternative names: Magnesium Transporter 1, Oligosaccharyl Transferase Subunit MAGT1, Implantation-Associated Protein, SLC58A1

Gene ID: 84061

NCBI Accession: NM_032121

UniProt: Q9H0U3

Pathways: Cell RedoxHomeostasis