MRGPRE antibody (AA 197-209)

Catalog No. ABIN7043319

This anti-MRGPRE antibody is a Rabbit Polyclonal antibody detecting MRGPRE in WB, IHC and IF. Suitable for Rat.

Quick Overview for MRGPRE antibody (AA 197-209) (ABIN7043319)

Target: MRGPRE (MAS-Related GPR, Member E (MRGPRE))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRGPRE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MRGPRE Antibody

Binding Specificity: AA 197-209

Purpose: A Rabbit Polyclonal Antibody to MRGPRE Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 10,13 amino acid residues identical

Characteristics: Anti-MRGPRE Antibody (ABIN7043319, ABIN7044604 and ABIN7044605) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRE from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RVERGPERHQPRG, corresponding to amino acid residues 197 - 209 of rat MRGPRE

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235909)

Blocking Peptide: (ABIN7235909)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MRGPRE

Target: MRGPRE (MAS-Related GPR, Member E (MRGPRE))

Alternative Name: MRGPRE

Background: Mas-related G-protein coupled receptor member E, GPCR MRGE, GPR167,The Mas-related G protein-coupled receptors (MRGPRs), formerly called Mas-related genes (MRGs) includes nine members: MrgprA, B, C, D, E, F, G, H, and the primate-specific MRGPRX1,2.MRGPRE consist of only one receptor per species and is conserved in rodents and primates2. MRGPRE structure contains a Cys-Cys bridge from transmembrane domain (TM) 3, an "ionic-lock" known to inhibit activation and activity in some class A GPCRs1,2.Human and mouse MRGPRE is highly expressed in neurons of the brain and mast cells. Transcripts are also found in medium and large diameter neurons of human dorsal root ganglia (DRG) sections and in other areas of the central nervous system, including cerebral cortex, hippocampus, spinal cord, and cerebellum. MRGPRE was also found in human, mouse, and rat placenta tissue and in enteric neurons1,2.Mouse lacking the MRGPRE protein showed decreased nociceptive behavior and significant deficits in the induction of mechanical allodynia after chronic constriction injury2.

Alternative names: MRGPRE, Mas-related G-protein coupled receptor member E, GPCR MRGE, GPR167

Gene ID: 404660

NCBI Accession: NM_001039165

UniProt: Q7TN40