MRGPRE antibody (3rd Intracellular Loop)

Catalog No. ABIN7886568

The Rabbit Polyclonal anti-MRGPRE antibody (ABIN7886568) specifically detects MRGPRE in WB, IHC and IF. The antibody is reactive with Rat samples.

Quick Overview for MRGPRE antibody (3rd Intracellular Loop) (ABIN7886568)

Target: MRGPRE (MAS-Related GPR, Member E (MRGPRE))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRGPRE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MRGPRE Antibody

Binding Specificity: 3rd Intracellular Loop, AA 197-209

Purpose: A Rabbit Polyclonal Antibody to MRGPRE Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 10,13 amino acid residues identical

Characteristics: Anti-MRGPRE Antibody (ABIN7043319, ABIN7044604 and ABIN7044605) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRE from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RVERGPERHQPRG, corresponding to amino acid residues 197 - 209 of rat MRGPRE

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235909)

Blocking Peptide: (ABIN7235909)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for MRGPRE

Target: MRGPRE (MAS-Related GPR, Member E (MRGPRE))

Alternative Name: MRGPRE

Background:

Synonyms: Mas-related G-protein coupled receptor member E, GPCR MRGE, GPR167

Description: The Mas-related G protein-coupled receptors (MRGPRs), formerly called Mas-related genes (MRGs) includes nine members: MrgprA, B, C, D, E, F, G, H, and the primate-specific MRGPRX1,2.MRGPRE consist of only one receptor per species and is conserved in rodents and primates2. MRGPRE structure contains a Cys-Cys bridge from transmembrane domain (TM) 3, an "ionic-lock" known to inhibit activation and activity in some class A GPCRs1,2.Human and mouse MRGPRE is highly expressed in neurons of the brain and mast cells. Transcripts are also found in medium and large diameter neurons of human dorsal root ganglia (DRG) sections and in other areas of the central nervous system, including cerebral cortex, hippocampus, spinal cord, and cerebellum. MRGPRE was also found in human, mouse, and rat placenta tissue and in enteric neurons1,2.Mouse lacking the MRGPRE protein showed decreased nociceptive behavior and significant deficits in the induction of mechanical allodynia after chronic constriction injury2.

Gene ID: 404660

NCBI Accession: NM_001039165

UniProt: Q7TN40