MC5 Receptor antibody (AA 224-236)

Catalog No. ABIN7043325

Product Details anti-MC5 Receptor Antibody

Target: MC5 Receptor (MC5R) (Melanocortin 5 Receptor (MC5R))

Binding Specificity: AA 224-236

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MC5 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Melanocortin Receptor 5

Specificity: 3rd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - 12,13 amino acid residues identical, human - 7

Characteristics: Anti-MC5 Receptor Antibody is directed against an epitope of the mouse melanocortin receptor 5. Anti-MC5 Receptor Antibody (ABIN7043325 and ABIN7044598)) can be used in western blot and immunohistochemical applications. It has been designed to recognize MC5R from mouse, human and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RYNSVRQRTSMKG, corresponding to amino acid residues 224-236 of mouse MC5R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-MR025

Blocking Peptide: BLP-MR025

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MC5 Receptor

Target: MC5 Receptor (MC5R) (Melanocortin 5 Receptor (MC5R))

Alternative Name: MC5R (MC5R Products)

Background: Melanocortin receptor 5, MC5R, MC5-R,Melanocortin receptor 5 (MC5R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G-Protein Coupled Receptor (GPCR) superfamily.The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC) 1,2,3.One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP) 1.All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC5R activation is α-MSH > ACTH = β-MSH >> γ-MSH while AGRP works as an endogenous antagonists of MC5R1,2,3.MC5R is mostly expressed in peripheral tissues such as skeletal muscle, bone marrow, skin tissues particularly in sebaceous glands and exocrine glands such as salivary glands1.The best understood function of Melanocortin Receptor 5 is in the regulation of exocrine glands since MC5R-deficient mice exhibit severely defective water repulsion and thermoregulation due to decreased production of sebaceous lipids4.

Alternative names: MC5 Receptor, Melanocortin receptor 5, MC5R, MC5-R

Gene ID: 17203

NCBI Accession: NM_005913

UniProt: P41149

Pathways: cAMP Metabolic Process