MC5 Receptor antibody (3rd Intracellular Loop)

Catalog No. ABIN7886562

The Rabbit Polyclonal anti-MC5 Receptor antibody (ABIN7886562) specifically detects MC5 Receptor in WB and IHC. The antibody is reactive with Mouse samples.

Quick Overview for MC5 Receptor antibody (3rd Intracellular Loop) (ABIN7886562)

Target: MC5 Receptor (MC5R) (Melanocortin 5 Receptor (MC5R))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MC5 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MC5 Receptor Antibody

Binding Specificity: 3rd Intracellular Loop, AA 224-236

Purpose: A Rabbit Polyclonal Antibody to Melanocortin Receptor 5

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - 12,13 amino acid residues identical, human - 7

Characteristics: Anti-MC5 Receptor Antibody is directed against an epitope of the mouse melanocortin receptor 5. Anti-MC5 Receptor Antibody (ABIN7043325 and ABIN7044598) can be used in western blot and immunohistochemical applications. It has been designed to recognize MC5R from mouse, human and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RYNSVRQRTSMKG, corresponding to amino acid residues 224-236 of mouse MC5R

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235932)

Blocking Peptide: (ABIN7235932)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for MC5 Receptor

Target: MC5 Receptor (MC5R) (Melanocortin 5 Receptor (MC5R))

Alternative Name: MC5R

Background:

Synonyms: Melanocortin receptor 5, MC5R, MC5-R

Description: Melanocortin receptor 5 (MC5R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G-Protein Coupled Receptor (GPCR) superfamily.The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC) 1,2,3.One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP) 1.All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC5R activation is α-MSH > ACTH = β-MSH >> γ-MSH while AGRP works as an endogenous antagonists of MC5R1,2,3.MC5R is mostly expressed in peripheral tissues such as skeletal muscle, bone marrow, skin tissues particularly in sebaceous glands and exocrine glands such as salivary glands1.The best understood function of Melanocortin Receptor 5 is in the regulation of exocrine glands since MC5R-deficient mice exhibit severely defective water repulsion and thermoregulation due to decreased production of sebaceous lipids4.

Gene ID: 17203

NCBI Accession: NM_005913

UniProt: P41149

Pathways: cAMP Metabolic Process