Melatonin Receptor 1B antibody (3rd Intracellular Loop)

Catalog No. ABIN7886564

The Rabbit Polyclonal anti-Melatonin Receptor 1B antibody is suitable to detect Melatonin Receptor 1B in samples from Mouse. It has been validated for WB, IHC, IF and IC.

Quick Overview for Melatonin Receptor 1B antibody (3rd Intracellular Loop) (ABIN7886564)

Target: Melatonin Receptor 1B (MTNR1B)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Melatonin Receptor 1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-Melatonin Receptor 1B Antibody

Binding Specificity: 3rd Intracellular Loop, AA 232-246

Purpose: A Rabbit Polyclonal Antibody to Melatonin Receptor Type 1B

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Unlikely to recognize human samples.

Predicted Reactivity: Rat - 14,15 amino acid residues identical

Characteristics: Anti-Melatonin Receptor 1B (MTNR1B) Antibody (ABIN7043327, ABIN7044601 and ABIN7044602) is a highly specific antibody directed against an epitope of mouse MT2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MT2 from rat and mouse samples. The antibody is unlikely to recognize the receptor in human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RKAKATRKLRLRPSD, corresponding to amino acid residues 232-246 of mouse MTNR1B

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:600-1:2000

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC|ICC

Negative Control: (ABIN7235940)

Blocking Peptide: (ABIN7235940)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Melatonin Receptor 1B

Target: Melatonin Receptor 1B (MTNR1B)

Alternative Name: MTNR1B

Background:

Synonyms: Melatonin receptor type 2, MT2, Mel1b receptor, Mel-1B-R

Description: Melatonin (N-acetyl-5-methoxytryptamine) is a product of tryptophan metabolism. It is synthesized in the pineal gland and is secreted to control the circadian rhythm1-3. Melatonin is also synthesized in the gastrointestinal tract, retina, skin and other tissues where it acts in a autocrine or paracrine manner. The role of melatonin in these tissues is independent of its role in the circadian rhythm, where it plays a role in energy metabolism, physiological growth, differentiation and responsiveness in stress stimuli1. The pleiotropic effects of melatonin have given rise to various therapeutic possibilities for this molecule. For example, anti-stress, sexual dysfunction, obesity, gallbladder stones1,4. To date, the only therapeutic uses for melatonin remain to treat sleep disorders, depression, migraine and headaches1.Melatonin exerts its effects through two G-protein coupled receptors (GPCRs), melatonin receptor type 1 and melatonin receptor type 2 (MT1 and MT2). Like all GPCRs, they have seven transmembrane domains and extracellular N-terminal and cytoplasmic C-terminal tails. The binding of melatonin to either receptor activates Gi, thereby activating PLC, thus increasing intracellular Ca2+ levels. Both receptors structurally bind melatonin in the same manner, although MT2 displays a much higher affinity for the hormone. Just like melatonin levels are detected in many tissues, the expression patterns of the two receptors are also quite broad. For example, MT1 is detected in the brain, retina and kidneys and MT2 is expressed in brain and in the retina1.MT1 is involved in sleep regulation and might also have effects on peripheral vasoconstriction. MT2 may play an important physiological role in the retina and might regulate body temperature1.

Gene ID: 244701

NCBI Accession: NM_005959

UniProt: Q8CIQ6