Mucolipin 3 antibody (Intracellular) (Atto 594)

Catalog No. ABIN7043339

The Rabbit Polyclonal anti-Mucolipin 3 antibody has been validated for IHC and IF. It is suitable to detect Mucolipin 3 in samples from Mouse.

Quick Overview for Mucolipin 3 antibody (Intracellular) (Atto 594) (ABIN7043339)

Target: Mucolipin 3 (Mcoln3)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mucolipin 3 antibody is conjugated to Atto 594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Mucolipin 3 Antibody (Atto 594)

Binding Specificity: AA 528-542, Intracellular

Purpose: A Rabbit Polyclonal Antibody to TRPML3 Channel Conjugated to the Fluorescent Dye ATTO-594

Specificity: Intracellular, C-terminus (cytoplasmic)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-TRPML3 (Mucolipin 3) Antibody (ABIN7043338 and ABIN7044043) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPML3 from human, rat and mouse samples. \nAnti-TRPML3 (Mucolipin 3)-ATTO Fluor-594 Antibody (ABIN7043339) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-TRPML3 (Mucolipin 3)-ATTO Fluor-594 Antibody has been tested in immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CKDLPNSGKYRLEDD, corresponding to amino acid residues 528-542 of mouse TRPML3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7235979)

Blocking Peptide: (ABIN7235979)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Mucolipin 3

Target: Mucolipin 3 (Mcoln3)

Alternative Name: MCOLN3

Background: Mcoln3,The endolysosome system takes part in important cellular functions such as membrane trafficking, protein transport, autophagy and signal transduction1. Endosomes result from endocytosis of the plasma membrane and lysosomes (which are derived from late endosomes) conatin mainly hydrolytic enzymes and generally have a low internal pH 1. Like the endoplasmic reticulum (ER), endolysosomes also store Ca2+ (luminal Ca2+ concentration: 0.5 mM)1,2, and similarly to Ca2+ release from the ER, Ca2+ from endolysosomes may also play an important role in various signaling events. To date such candidates include members of the TRP super-family of ion channels and the two-pore Ca2+ channels (TPCs)1,3,4.TRPMLs, also termed mucolipins, are members of the TRP channels. In mammals, three TRPMLs are known to date (TRPML1-3 or MCOLN1-3). They are all localized to endolysosomes, although when over expressed in heterologous systems, TRPML3 is found on the plasma membrane1,5. These channels are Ca2+ permeable and display inward rectifying current properties1,5. Like all members of this family, TRPMLs have six transmembrane domains and intracellular N- and C-termini (relatively short tails compared to other members). They are characterized by an exceptionally large extracellular (luminal) loop between transmembrane domains 1 and 2, and N-glycosylation sites are present in the first extracellular (luminal) loop5.In mammals, TRPML1 is expressed in a ubiquitous manner and shows highest expression in the brain, kidney, spleen, liver and heart1,6. TRPML2 and TRPML3 are less widely expressed. Interestingly, in mouse, two splice variants exist for TRPML2. The shorter variant is more broadly expressed and is dominant over the longer variant in the thymus, spleen and kidney1,7. TRPML3 is highly detected in the thymus, lung, kidney, spleen and eye1,7,8, some epithelial cells1,9 and brain10.Pathologies related to these channels include type IV mucolipidosis, a neurodegenetative disease characterized by retardation and retinal degeneration caused by a loss of function mutation in the gene encoding TRPML1. In contrast, a gain of function mutation in TRPML3, in mice, causes deafness, and pigmentation defects11.

Alternative names: TRPML3 (Mucolipin 3), Mcoln3

Gene ID: 171166

NCBI Accession: NM_018298

UniProt: Q8R4F0

Pathways: Sensory Perception of Sound