PVRL2 antibody (Extracellular)

Catalog No. ABIN7043340

Product Details anti-PVRL2 Antibody

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Binding Specificity: AA 148-161, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PVRL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Nectin-2/PVRL2

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 10,14 amino acid residues identical

Characteristics: Anti-Nectin-2/PVRL2 (extracellular) Antibody is directed against an epitope of mouse Poliovirus receptor-related protein 2. Anti-Nectin-2/PVRL2 (extracellular) Antibody (ABIN7043340, ABIN7044694 and ABIN7044695) can be used in western blot, immunohistochemistry, and flow cytometry applications. It has been designed to recognize Nectin-2 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RVIAQPENHAEAQE, corresponding to amino acid residues 148-161 of mouse Nectin-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: (ABIN7235990)

Blocking Peptide: (ABIN7235990)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PVRL2

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Alternative Name: NECTIN2 (PVRL2 Products)

Background: Poliovirus receptor-related protein 2, Herpes virus entry mediator B, PRR2, PVRR2, CD112,The Nectin family is known as cell-cell adhesion molecules and comprises four members in this family: nectin-1, nectin-2, nectin-3 and nectin-4 which are encoded by PVRL1, PVRL2, PVRL3, and PVRL4 genes respectively1. They are Ca2+-independent immunoglobulin-like CAMs and are able to trans-interact1.They are localized at adherens junctions between two neighboring cells and binds to afadin through their C-terminal region1.Nectins, apart from a secreted nectin-1γ, exhibit three immunoglobulin-like loops (one V type and two C2 type) in their extracellular region, a single trans membrane segment and a cytoplasmic tail. Nectins first form a cis-homo-dimer which later on undergoes lateral cluster formation on the cell surface1. Nectins regulate a variety of cellular functions in addition to cell-cell junction formation such as cell polarity, movement, proliferation, differentiation, and survival1.The PVRL2 gene (which encodes nectin-2) encodes two mRNA isoforms of 3 kb and 4.4 kb both widely expressed in various human tissues including hematopoietic cells of the myelo-monocytic and megakaryocytic lineages and detected also on the surface of endothelial cells2.Nectin-2 acts as mediator of herpes simplex virus invasion into host cell. A single nucleotide polymorphism in PVRL2 shows an association with later onset of Alzheimers Disease3.

Alternative names: Nectin-2/PVRL2, Poliovirus receptor-related protein 2, Herpes virus entry mediator B, PRR2, PVRR2, CD112

Gene ID: 19294

NCBI Accession: NM_002856

UniProt: P32507

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization