Netrin 1 antibody (Secreted)

Catalog No. ABIN7043349

Product Details anti-Netrin 1 Antibody

Target: Netrin 1 (NTN1)

Binding Specificity: AA 181-195, Secreted

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Netrin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Netrin-1

Specificity: Secreted

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Netrin-1 Antibody (ABIN7043349, ABIN7044726 and ABIN7044727)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Netrin-1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CRKMYNRPHRAPITK, corresponding to amino acid residues 181 - 195 of rat Netrin-1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NR121

Blocking Peptide: BLP-NR121

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Netrin 1

Target: Netrin 1 (NTN1)

Alternative Name: NTN1 (NTN1 Products)

Background: NTN1, MRMV4,Netrins are a family comprised of five members: three secreted proteins (netrins 1, 3 and 4), and two glycosylphosphatidylinositol (GPI)-anchored membrane proteins, netrins G1 and G21. Netrin-1 is a diffusible long-range axonal guidance cue expressed in floor plate cells of the central nervous system. It is secreted in order to direct exons to their target1-3. Axon guidance by netrin-1 is implicated in multiple developing brain regions and developing neuronal types. It also plays a role in axon branching, synaptogenesis, cell migration, cell survival, and axon regeneration4.

Alternative names: NTN1, MRMV4

Gene ID: 114523

NCBI Accession: NM_004822

UniProt: Q924Z9

Pathways: Regulation of Cell Size