NCAM2 antibody (Extracellular)

Catalog No. ABIN7043352

Product Details anti-NCAM2 Antibody

Target: NCAM2 (Neural Cell Adhesion Molecule 2 (NCAM2))

Binding Specificity: AA 635-650, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCAM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Neural Cell Adhesion Molecule 2

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-NCAM2 (extracellular) Antibody (ABIN7043352, ABIN7044688 and ABIN7044689) is a highly specific antibody directed against mouse Neural cell adhesion molecule 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NCAM2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEDQWLEKKVQGNKDH, corresponding to amino acid residues 635-650 of mouse NCAM-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236022)

Blocking Peptide: (ABIN7236022)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NCAM2

Target: NCAM2 (Neural Cell Adhesion Molecule 2 (NCAM2))

Alternative Name: NCAM2 (NCAM2 Products)

Background: Neural cell adhesion molecule 2, NCAM-2, R4B12, Ocam, Rncam, NCAM21, Neural cell adhesion molecule RB-8,Neural cell adhesion molecule 2 (NCAM-2), a protein also known as olfactory cell adhesion molecule (OCAM), belongs to the immunoglobulin superfamily of cell adhesion molecules that participate in homophilic trans-interactions. NCAM-2 is highly expressed in the nervous system and participates in numerous cellular events, such as survival, migration, and differentiation, neurite outgrowth, and formation and plasticity of synapses1,2. The NCAM-2 structure consists of five immunoglobulin (Ig) domains followed by two fibronectin-type 3 (Fn3) domains and eight potential sites for N-linked glycosylation3.Overexpression of NCAM-2 is believed to be a contributing factor to Down syndrome. There is also a genetic association between NCAM-2 and Alzheimer's disease, where NCAM-2 is involved in the pathogenic pathway to the senile plaques that concentrate in the Alzheimer's disease brain1.During human embryonic development, NCAM-2 is expressed in several tissues, including lung, liver, and kidney with the highest expression in the brain. NCAM-2 expression level rises around postnatal day 21 and remains high during adulthood. Studies conducted on NCAM-2 deficient mice show that the protein is important for the development of the brain and the olfactory system1.

Alternative names: NCAM2, Neural cell adhesion molecule 2, NCAM-2, R4B12, Ocam, Rncam, NCAM21, Neural cell adhesion molecule RB-8

Gene ID: 17968

NCBI Accession: NM_004540

UniProt: O35136