NCAM2 antibody (Extracellular, N-Term)

Catalog No. ABIN7043352

The Rabbit Polyclonal anti-NCAM2 antibody (ABIN7043352) specifically detects NCAM2 in WB, IHC, FACS, IF and LCI. The antibody is reactive with Mouse samples.

Quick Overview for NCAM2 antibody (Extracellular, N-Term) (ABIN7043352)

Target: NCAM2 (Neural Cell Adhesion Molecule 2 (NCAM2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCAM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details anti-NCAM2 Antibody

Binding Specificity: AA 635-650, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Neural Cell Adhesion Molecule 2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-NCAM2 (extracellular) Antibody (ABIN7043352, ABIN7044688 and ABIN7044689) is a highly specific antibody directed against mouse Neural cell adhesion molecule 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NCAM2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KEDQWLEKKVQGNKDH, corresponding to amino acid residues 635-650 of mouse NCAM-2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236022)

Blocking Peptide: (ABIN7236022)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NCAM2

Target: NCAM2 (Neural Cell Adhesion Molecule 2 (NCAM2))

Alternative Name: NCAM2

Background:

Synonyms: Neural cell adhesion molecule 2, NCAM-2, R4B12, Ocam, Rncam, NCAM21, Neural cell adhesion molecule RB-8

Description: Neural cell adhesion molecule 2 (NCAM-2), a protein also known as olfactory cell adhesion molecule (OCAM), belongs to the immunoglobulin superfamily of cell adhesion molecules that participate in homophilic trans-interactions. NCAM-2 is highly expressed in the nervous system and participates in numerous cellular events, such as survival, migration, and differentiation, neurite outgrowth, and formation and plasticity of synapses1,2. The NCAM-2 structure consists of five immunoglobulin (Ig) domains followed by two fibronectin-type 3 (Fn3) domains and eight potential sites for N-linked glycosylation3.Overexpression of NCAM-2 is believed to be a contributing factor to Down syndrome. There is also a genetic association between NCAM-2 and Alzheimer's disease, where NCAM-2 is involved in the pathogenic pathway to the senile plaques that concentrate in the Alzheimer's disease brain1.During human embryonic development, NCAM-2 is expressed in several tissues, including lung, liver, and kidney with the highest expression in the brain. NCAM-2 expression level rises around postnatal day 21 and remains high during adulthood. Studies conducted on NCAM-2 deficient mice show that the protein is important for the development of the brain and the olfactory system1.

Gene ID: 17968

NCBI Accession: NM_004540

UniProt: O35136