Neurexin 1 antibody (Extracellular) (Atto 488)

Catalog No. ABIN7043358

This anti-Neurexin 1 antibody is a Rabbit Polyclonal antibody detecting Neurexin 1 in IHC and IF. Suitable for Rat.

Quick Overview for Neurexin 1 antibody (Extracellular) (Atto 488) (ABIN7043358)

Target: Neurexin 1 (NRXN1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurexin 1 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Neurexin 1 Antibody (Atto 488)

Binding Specificity: AA 546-560, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Neurexin 1α Conjugated to the Fluorescent Dye ATTO-488

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Neurexin 1α (extracellular) Antibody (ABIN7043357, ABIN7044676 and ABIN7044677) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and live cell imaging applications. It has been designed to recognize Nrxn1α from rat, mouse and human samples. \nAnti-Neurexin 1α (extracellular)- Antibody (ABIN7043358) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Neurexin 1α (extracellular)- Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KPRHQKDAKHPQMIK, corresponding to amino acid residues 546-560 of rat Neurexin 1alpha

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582041)

Blocking Peptide: (ABIN7236037)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neurexin 1

Target: Neurexin 1 (NRXN1)

Alternative Name: NRXN1

Background: Nrxn1, Neurexin I-alpha,Neurexins (NRXNs) are a family of transmembrane, synaptic adhesion molecules. NRXNS were identified as receptors for α-latrotoxin, a presynaptic toxin that triggers massive neurotransmitter release1. Neurexins are largely presynaptic proteins that form a trans-synaptic cell-adhesion complex with postsynaptic neuroligins2. They are encoded by three genes (NRXN1, NRXN2 and NRXN3), each using an upstream promoter to produce the longer α-isoform (α-NRXNs) and a downstream promoter to generate a shorter β-isoform (β- NRXNs).The α- and β-isoforms of each neurexin are single-pass transmembrane proteins maintaining identical transmembrane and intracellular domains but having distinct extracellular domains. NRXNs in neurons localize to the presynaptic membrane and bind trans-synaptically to postsynaptic adhesion molecules and receptors3. Neurexins are expressed in all neurons, and are subject to extensive alternative splicing, generating >1,000 splice variants, some of which exhibit highly regulated developmental and spatial expression patterns4. The extracellular domain of neurexin 1α (NRXN1α) is composed of three neurexin repeats (I, II, and III), which each contains the modules LNS-EGF-LNS. NRXN1α binds endogenous ligands including neuroligins, LRRTM family members, neurexophilin, α-dystroglycan, and GABA(A) receptors.NRXN1α gene mutations have been identified in neuropsychiatric diseases including Schizophrenia and Autism Spectrum Disorder5.

Alternative names: Neurexin 1alpha, Neurexin I-alpha, Nrxn1

Gene ID: 60391

NCBI Accession: NM_001135659

UniProt: Q63372

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development