Neurexin 3 antibody (Extracellular)

Catalog No. ABIN7043359

Product Details anti-Neurexin 3 Antibody

Target: Neurexin 3 (NRXN3)

Binding Specificity: AA 39-52, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurexin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Neurexin 3α

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Designed to recognize Neurexin 3α and not Neurexin 3β.

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Neurexin 3alpha (extracellular) Antibody is directed against an epitope of rat Neurexin 3α. Anti-Neurexin 3α (extracellular) Antibody (ABIN7043359, ABIN7044678 and ABIN7044679)) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is therefore, highly suited to recognize Nrxn3α in living cells. It has been designed to recognize Nrxn3α from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RYLRWDASTRSDLS, corresponding to amino acid residues 39-52 of rat Nrxn3alpha

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NR033

Blocking Peptide: BLP-NR033

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neurexin 3

Target: Neurexin 3 (NRXN3)

Alternative Name: NRXN3 (NRXN3 Products)

Background: Nrxn3, NRX3A, Neurexin III-alpha,Neurexins (NRXNs) are a family of transmembrane, synaptic adhesion molecules. NRXNS were identified as receptors for α-latrotoxin, a presynaptic toxin that triggers massive neurotransmitter release1. Neurexins are largely presynaptic proteins that form a trans-synaptic cell-adhesion complex with postsynaptic neuroligins2. They are encoded by three genes (NRXN1, NRXN2 and NRXN3), each using an upstream promoter to produce the longer α-isoform (α-NRXNs) and a downstream promoter to generate a shorter β-isoform (β-NRXNs).The α-isoforms and β-isoforms of each neurexin are single-pass transmembrane proteins maintaining identical transmembrane and intracellular domains but having distinct extracellular domains. NRXNs in neurons localize to the presynaptic membrane and bind trans-synaptically to postsynaptic adhesion molecules and receptors3. Neurexins are expressed in all neurons, and are subject to extensive alternative splicing, generating >1,000 splice variants, some of which exhibit highly regulated developmental and spatial expression patterns4.The structure of α-Neurexins is composed of 6 LNS domains (laminin, neurexin, sex-hormone-binding protein domain) and 3 EGF-like domains. In the mammalian brain they are known to interact with neuroligins, dystroglycan and neurexophilins. Neurexin 3α splicing can give rise to secreted and transmembrane forms of the protein. The biological function of the secreted form remains unknown5.Changes in Neurexin 3α are associated with autism, drug addiction and obesity6.

Alternative names: Neurexin 3alpha, Nrxn3, NRX3A, Neurexin III-alpha

Gene ID: 116508

NCBI Accession: NM_001105250

UniProt: Q07310