NIPAL4 antibody (2nd Extracellular Loop)

Catalog No. ABIN7043394

The Rabbit Polyclonal anti-NIPAL4 antibody (ABIN7043394) specifically detects NIPAL4 in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for NIPAL4 antibody (2nd Extracellular Loop) (ABIN7043394)

Target: NIPAL4 (NIPA-Like Domain Containing 4 (NIPAL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIPAL4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-NIPAL4 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 242-256

Purpose: A Rabbit Polyclonal Antibody to Magnesium Transporter NIPA4 

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: rat - 12,15 amino acid residues identical,Mouse - 13

Characteristics: Anti-NIPAL4 (extracellular) Antibody is directed against an epitope of human magnesium transporter NIPA4. Anti-NIPAL4 (extracellular) Antibody (ABIN7043394, ABIN7044847 and ABIN7044848) can be used in western blot and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the transporter in living cells. It has been designed to recognize NIPAL4 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EKVTTIMEMASKMKD, corresponding to amino acid residues 242-256 of human magnesium transporter NIPA4

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236151)

Blocking Peptide: (ABIN7236151)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NIPAL4

Target: NIPAL4 (NIPA-Like Domain Containing 4 (NIPAL4))

Alternative Name: NIPAL4

Background:

Synonyms: Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4

Description: The NIPA family is currently comprised of 4 members- NIPA1-4. These members have a molecular similarity of 40 % . NIPA proteins are integral membrane proteins which function as Mg2+ transporters.The NIPA4 gene in mice is located on chromosome 11B1.1 and the human corresponding gene, hIchthyin is located on chromosome 5q33.3. Both genes share an 85 % amino acid similarity. Interestingly, NIPA2-4 show greater common identity in their ancestral invertebrate polypeptides than they do to NIPA1 suggesting different physiological roles. NIPA4 mediates Mg2+ uptake with a Km of 0.36 mM but unlike NIPA2, which is selective for Mg2+, it has been found to transport other cations as well.Mutations in the hIchthyin gene provide the basis of one form of autosomal-recessive congenital ichthyosis (ARCI). ARCI is a heterogenous group of disorders with abnormal differentiation and desquamation of the epidermis, leading to widespread scaling and erythema of the skin. To this day six homozygous mutations have been found, including one nonsense and five missense mutations, in a large number of consanguineous families presenting with ichthyosis. The function of ichthyin in skin differentiation is not fully understood but it has been suggested that because lipid dysfunctions are involved with the other ARCI disorders ichthyin might perform as a membrane receptor for trioxilins of the hepoxilin pathway.Although some NIPA proteins have been implicated as candidates for prader-willi syndrome their involvement remains unclear. Currently there is no evidence that NIPA4 is directly involved in the many complexities of the syndrome1.

Gene ID: 348938

NCBI Accession: NM_001099287

UniProt: Q0D2K0