OPRK1 antibody (Extracellular, N-Term)

Catalog No. ABIN7886828

The Rabbit Polyclonal anti-OPRK1 antibody is suitable to detect OPRK1 in samples from Rat. It has been validated for WB, IHC, FACS and LCI.

Quick Overview for OPRK1 antibody (Extracellular, N-Term) (ABIN7886828)

Target: OPRK1 (Opioid Receptor, kappa 1 (OPRK1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPRK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-OPRK1 Antibody

Binding Specificity: AA 39-55, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to κ-Opioid Receptor (OPRK1)

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: human - 15,17 amino acid residues identical,Mouse -16

Characteristics: Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody is directed against an epitope of the rat κ-opioid receptor. Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody (ABIN7043402, ABIN7044889 and ABIN7044890) can be used in western blot analysis. The antibody recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. The antibody was designed to recognize κ-opioid receptor from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)NGSVGSEDQQLEPAHIS, corresponding to amino acid residues 39-55 of rat kappa-Opioid receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC

Negative Control: (ABIN7236171)

Blocking Peptide: (ABIN7236171)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for OPRK1

Target: OPRK1 (Opioid Receptor, kappa 1 (OPRK1))

Alternative Name: OPRK1

Background:

Synonyms: Kappa-type opioid receptor, KOR-1, Opiate receptor kappa 1

Description: Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (μ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor1. Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic μ, δ, and κ receptors2.The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins1.All three classic opioid receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the μ-opioid receptor, whereas μ-, δ-, and κ-receptors participate in the control of pain at the spinal level3. The opioid receptors also mediate the mood-altering properties of opioids4.κ-Opioid Receptor is activated by the endogenous peptide dynorphin. Malfunction of their signaling may be involved in epilepsy, addiction, depression, schizophrenia, and chronic pain5.κ-Opioid Receptor is localized to a number of regions in the brain, as well as the spinal cord, the enteric nervous system and the digestive system5.

Gene ID: 29335

NCBI Accession: NM_000912

UniProt: P34975