ORAI2 antibody (Intracellular)

Catalog No. ABIN7043414

Product Details anti-ORAI2 Antibody

Target: ORAI2 (ORAI Calcium Release-Activated Calcium Modulator 2 (ORAI2))

Binding Specificity: AA 16-32, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORAI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Orai2 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - identical

Characteristics: Anti-Orai2 Antibody (ABIN7043414, ABIN7044022 and ABIN7044023) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Orai2 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CPEPGHKGMDYRDWVRR, corresponding to amino acid residues 16-32 of mouse Orai2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100-1:300

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: (ABIN7236183)

Blocking Peptide: (ABIN7236183)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ORAI2

Target: ORAI2 (ORAI Calcium Release-Activated Calcium Modulator 2 (ORAI2))

Alternative Name: ORAI2 (ORAI2 Products)

Background: TMEM142B,Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx, either by the calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores, has become of great interest.Recently, several key players of the store-operated complex have been identified: the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum Ca2+ sensor.3,4 Orai1, Orai2, and Orai3 are all capable of forming store-operated channels.5Co-expresssion of Orai2 and STIM1 was shown to produce currents that appear similar but smaller than those produced by co-expression of Orai1 and STIM1.6Orai2 transcripts are predominantly expressed in kidney, lung, and spleen, and it appears that Orai2 is the only Orai isoform with multiple transcripts.7

Alternative names: Orai2, TMEM142B

Gene ID: 269717

NCBI Accession: NM_032831

UniProt: Q8BH10