SLC22A3 antibody (Extracellular)

Catalog No. ABIN7043416

Product Details anti-SLC22A3 Antibody

Target: SLC22A3 (OCT3) (Organic Cation Transporter 3 (OCT3))

Binding Specificity: AA 83-96, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC22A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Organic Cation Transporter 3

Specificity: 1st extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Rat,mouse - 13,14 amino acid residues identical

Characteristics: Anti-SLC22A3 (extracellular) Antibody (ABIN7043416, ABIN7044223 and ABIN7044224)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Organic cation transporter 3 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ERRGQGH(S)HRYLLE, corresponding to amino acid residues 83-96 of rat OCT3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CT013

Blocking Peptide: BLP-CT013

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC22A3

Target: SLC22A3 (OCT3) (Organic Cation Transporter 3 (OCT3))

Alternative Name: SLC22A3 (OCT3 Products)

Background: OCT3, Organic cation transporter 3,Organic cation transporter 3 (SLC22A3) belongs to the SLC22A gene family. Organic cation transporters (OCTs) are membrane transport proteins involved in many metabolic processes and detoxification. The transporters are responsible for the uptake and intracellular inactivation of numerous endogenous and exogenous substrates including norepinephrine, epinephrine, dopamine, histamine and serotonin. They are also responsible for the uptake of a variety of anticancer drugs1,2. OCT structure is suggested to have 12 transmembrane domains and a large hydrophobic cleft capable of accommodating a wide variety of chemical species3.In humans, OCT3 is expressed in many tissues, abundantly in the placenta, heart, liver and skeletal muscle. It is also expressed in astrocytes, intestinal epithelium neurons, glia, ependymal cells, and blood-cerebrospinal fluid barrier in choroid plexus epithelial cells. The protein is also widely distributed in the brain. OCT expression is detected in several cancer cell lines and tumor tissue samples1-3.

Alternative names: SLC22A3, OCT3, Organic cation transporter 3

Gene ID: 29504

NCBI Accession: NM_021977

UniProt: O88446