OXGR1 antibody (C-Term, Intracellular)

Catalog No. ABIN7886831

The Rabbit Polyclonal anti-OXGR1 antibody (ABIN7886831) specifically detects OXGR1 in WB, IF and IHC. The antibody is reactive with Mouse samples.

Quick Overview for OXGR1 antibody (C-Term, Intracellular) (ABIN7886831)

Target: OXGR1 (Oxoglutarate (Alpha-Ketoglutarate) Receptor 1 (OXGR1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OXGR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-OXGR1 Antibody

Binding Specificity: AA 319-332, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Oxoglutarate Receptor 1

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Rat - 12, human - 9

Characteristics: Anti-OXGR1/GPR99 Antibody (ABIN7043418, ABIN7044895 and ABIN7044896) is a highly specific antibody directed against an epitope of the mouse Oxoglutarate receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize OXGR1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CKASGDLEQGKKDS, corresponding to amino acid residues 319-332 of mouse OXGR1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:600

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236198)

Blocking Peptide: (ABIN7236198)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for OXGR1

Target: OXGR1 (Oxoglutarate (Alpha-Ketoglutarate) Receptor 1 (OXGR1))

Alternative Name: OXGR1

Background:

Synonyms: Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, aKGR, GPR80, P2RY15, P2Y15

Description: Oxoglutarate receptor 1 (OXGR1) is a receptor for α-ketoglutarate (αKG), a metabolite that is a citric acid cycle intermediate and a glutamine catabolism by-product1. αKG also serves as a metabolic marker and its interaction with OXGR1 has been shown to mediate the adaptive regulation of HCO3- secretion and NaCl reabsorption under acid-base stress3.OXGR1 belongs to the G-protein coupled receptor (GPCRs) protein family. Members of this group contain seven trans-membrane domains and respond to a large variety of ligands and thereby affect key cellular functions that are crucial for cell survival or proliferation2. OXGR1 belongs to a subset of metabolic GPCRs, along with receptors for succinate (GPR91), lactate (GPR81), fatty acids (FFAR) and many more3.OXGR1 is expressed in the kidney3,4 and in the heart, where it affects the contractile function, consistent with the finding that α-ketoglutarate levels are high in heart failure patients5.Because of its major role in maintaining cellular balance and its role in heart contractile activity, identification of molecules that can specifically activate or inhibit this receptor may be very useful in the development of novel therapeutic approach for pathological cardiac hypertrophy5.

Gene ID: 239283

NCBI Accession: NM_080818

UniProt: Q6IYF8