PTH1R antibody (Extracellular)

Catalog No. ABIN7043429

Product Details anti-PTH1R Antibody

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Binding Specificity: AA 241-255, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTH1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Parathyroid Hormone 1 Receptor

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-PTH1R (extracellular) Antibody (ABIN7043429, ABIN7045112 and ABIN7045113)) is a highly specific antibody directed against an epitope of the rat Parathyroid hormone 1 receptor. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize PTH1R from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DAVLYSGFTLDEAER, corresponding to amino acid residues 241-255 of rat PTH1R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:60

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IFC

Negative Control: BLP-PR051

Blocking Peptide: BLP-PR051

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PTH1R

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Alternative Name: PTH1R (PTH1R Products)

Background: Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1,The parathyroid hormone receptor type 1 (PTHR1) is bound and activated by two peptide hormones: the parathyroid hormone (PTH) secreted by the parathyroid gland and by parathyroid hormone related peptide (PTHrP) which is produced locally in many tissues. Although these two peptides bind the same receptor, they exert different actions. PTH regulates the concentrations of Ca2+, phosphate ions and vitamin D in the blood, stimulates bone formation/resorption and is distributed to the kidneys where it regulates Ca2+ and phosphate transport, and is also detected in the liver1-3. PTHrP, on the other hand, plays a critical role early in development of bone, heart, mammary glands and other tissues, and is detected in the circulation only during lactation and secreted by some tumors1,2,4,5. PTHR1 is obviously localized to all areas and tissues where PTH and PTHrP are known to exert their effects (i.e. bone, kidney, blood, heart, etc.).PTHR1 belongs to the superfamily of G-protein coupled receptors. Like all members, PTHR1 has seven transmembrane spanning domains, an extracellular N-terminus and an intracellular C-terminal tail. Upon activation, the receptor couples to either Gs and Gq thereby activating adenylate cyclase and phospholipase C (PLC) respectively. In osteosarcoma cells, PTHR1 can also couple Gα2,6,7.PTH is being used today to treat osteoporosis, as a factor promoting bone formation. Mutations in PTHR1 are associated with Jansen's disease (hypercalcemia and dwarfism) and Eiken syndrome (cartilage tumors of bone)2,8-10.

Alternative names: PTH1R, Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1

Gene ID: 56813

NCBI Accession: NM_000316

UniProt: P25961

Pathways: Regulation of Carbohydrate Metabolic Process