PTH2R antibody (Extracellular, N-Term)

Catalog No. ABIN7887004

The Rabbit Polyclonal anti-PTH2R antibody (ABIN7887004) specifically detects PTH2R in WB. The antibody is reactive with Rat samples.

Quick Overview for PTH2R antibody (Extracellular, N-Term) (ABIN7887004)

Target: PTH2R (Parathyroid Hormone 2 Receptor (PTH2R))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTH2R antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PTH2R Antibody

Binding Specificity: AA 125-137, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 11,13 amino acid residues identical

Characteristics: Anti-PTH2R (extracellular) Antibody (ABIN7043430, ABIN7045114 and ABIN7045115) is a highly specific antibody directed against an extracellular epitope of the rat Parathyroid hormone 2 receptor. The antibody can be used in western blot analysis. It has been designed to recognize PTH2 receptor from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CFLQPDINIGKQE, corresponding to amino acid residues 125-137 of rat PTH2R

Isotype: IgG

Application Details

Application Notes:

WB: 1:600

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236237)

Blocking Peptide: (ABIN7236237)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for PTH2R

Target: PTH2R (Parathyroid Hormone 2 Receptor (PTH2R))

Alternative Name: PTH2R

Background:

Synonyms: Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2

Description: PTH (parathyroid hormone) regulates Ca2+ and phosphate homeostasis through its action on specific receptors in kidney and bone. There are two known PTH receptors in humans, PTH receptor type 1 (PTH1 receptor) and PTH receptor type 2 (PTH2 receptor)1.The PTH2 receptor is a member of a class B subfamily of G-protein-coupled receptors that includes the receptors for the glucagon-GHRH-VIP family of peptides (glucagon, GLP-I, GIP, GHRH, VIP, secretin, PACAP) and for calcitonin and CRF2. PTH2 receptor shares the same basic structure of the GPCR superfamily of proteins. A defining feature of the family B receptors is the relatively long extracellular N-terminal domain, which comprises about 150 amino acids and is important for ligand binding. The seven transmembrane domains are believed to be arranged in a circular or oval configuration, as seen in rhodopsin. The transmembrane domains are connected by three extracellular and three intracellular loops, and a C-terminal tail of about 130 amino acids extends intracellularly3.Northern blots of human messenger RNA (mRNA) show that the PTH2 receptor is most highly expressed in the central nervous system, preferentially in the hypothalamic regions and is also detected in the pancreas, testis, placenta, and lung4.Recent data support that PTH2 receptor is involved in hypothalamic releasing-factor secretion and pain5.

Gene ID: 81753

NCBI Accession: NM_005048

UniProt: P70555

Pathways: cAMP Metabolic Process