Plexin A3 antibody (Extracellular, N-Term)
Quick Overview for Plexin A3 antibody (Extracellular, N-Term) (ABIN7887021)
Target
See all Plexin A3 (PLXNA3) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 440-453, Extracellular, N-Term
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Purpose
- A Rabbit Polyclonal Antibody to Plexin-A3
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Cross-Reactivity
- Human, Mouse, Rat
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Predicted Reactivity
- Rat - identical, human - 12,14 amino acid residues identical
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Characteristics
- Anti-Plexin-A3 (extracellular) Antibody (ABIN7043438, ABIN7045134 and ABIN7045135) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize PLXNA3 from rat, mouse, and human samples.
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Purification
- Affinity purified on immobilized antigen.
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Immunogen
- (C)KVRVDGSQDAQLYE, corresponding to amino acid residues 440-453 of mouse PLXNA3
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Isotype
- IgG
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anti-Plexin A3 (PLXNA3) (AA 1050-1100) antibody
PLXNA3 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
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Application Notes
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WB: 1:200
FC: 2.5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: 1:200
IP: The optimal concentration should be determined by the user
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Comment
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Cited Application: IHC
Negative Control: (ABIN7236268)
Blocking Peptide: (ABIN7236268)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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Concentration
- 0.8 mg/mL
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Buffer
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- Plexin A3 (PLXNA3)
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Alternative Name
- PLXNA3
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Background
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Synonyms: PLXNA3, PLXN3, Semaphorin receptor SEX
Description: Plexins are type 1 transmembrane (TM) receptors acting as key regulatory proteins in a wide variety of developmental, regenerative and pathological processes. They are involved in guidance and motility of vascular, lymphatic vessel, and neuron growth, and in cancer metastasis.Plexin family includes 9 proteins: Plexin-A1-4, -B1-3, -C1 and -D1. Class A Plexins interact with the secreted class 3 Semaphorins, and require Neuropilins (nrps) as co-receptors.Plexin protein signaling results in cytosolic GTPase-activating protein activity including direct interactions with Rho GTPases and transient/catalytic interactions with Ras GTPases1,2.Plexin structure consists of an extracellular semaphorin domain, three Plexin-Semaphorin-Integrin (PSI) domains, and three immunoglobulin, Plexin, and transcription factor (IPT) domains. In addition, a single-spanning transmembrane domain, and a cytosolic region (CYTO) homologous with Ras GTPase-activating proteins (GAPs)2. The transmembrane region of Plexin-A3 is characterized by poly-glycine usually used for close helix-helix packing and aromatic residues that distort helix conformation and association. Plexin-A family members only have 22 residues spanning the membrane1.Mutations in Plexins have been reported in melanoma, lung, breast, pancreatic, and prostate cancers, strongly suggesting that their signaling may play a role in cancer development. Plexins are widely expressed in the nervous system and in the developing cerebral cortex2.
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Gene ID
- 18846
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NCBI Accession
- NM_017514
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UniProt
- P70208
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Pathways
- Regulation of Cell Size
Target
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