KCNK9 antibody (Extracellular)

Catalog No. ABIN7043456

The Rabbit Polyclonal anti-KCNK9 antibody has been validated for WB, IHC and IF. It is suitable to detect KCNK9 in samples from Rat.

Quick Overview for KCNK9 antibody (Extracellular) (ABIN7043456)

Target: KCNK9 (Potassium Channel, Subfamily K, Member 9 (KCNK9))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-KCNK9 Antibody

Binding Specificity: AA 57-73, Extracellular

Purpose: A Rabbit Polyclonal Antibody to KCNK9 Channel 

Specificity: Extracellular, near the P1 loop

Cross-Reactivity: Human, Rat

Predicted Reactivity: Human - 15,17 amino acid residues identical

Characteristics: Anti-KCNK9 (extracellular) Antibody (ABIN7043456, ABIN7044953 and ABIN7044954) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize from rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DDYQQLELVILQSEPHR, corresponding to amino acid residues 57-73 of rat KCNK9

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: ICC

Negative Control: (ABIN7236330)

Blocking Peptide: (ABIN7236330)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK9

Target: KCNK9 (Potassium Channel, Subfamily K, Member 9 (KCNK9))

Alternative Name: KCNK9

Background: Potassium channel subfamily K member 9, K2P9.1, TWIK-related acid-sensitive K+ channel 3, Two pore K+ channel KT3.2,KCNK9 (also named TASK3 or K2P9.1) is a member of the 2-pore (2P) domain K+ channels family that probably assemble as dimers to constitute a functional channel.1 These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The channels are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, etc.2 The physiological role of the K2P channels is not yet clear, though they were proposed to participate in breathing, aldosterone secretion and anesthetic-mediated neuronal activity.In human, expression of KCNK9 is restricted in brain and its expression overlaps with the structurally related KCNK3 (TASK1, K2P3.1) channel. In rat, it is more widely expressed in other tissues as well as in brain. Unexpectedly, KCNK9 was found to be over-expressed between 5 to 100-fold in 44 % of breast cancer tumors tested. Transfection and over-expression of KCNK9 in cell lines promotes tumor growth and confers resistance to hypoxia and serum deprivation.3 Recently, KCNK9 was found to play an important role in K+-dependent apoptosis of cerebellar granules.4

Alternative names: KCNK9 (TASK-3), Potassium channel subfamily K member 9, K2P9.1, TWIK-related acid-sensitive K+ channel 3, Two pore K+ channel KT3.2

Gene ID: 84429

NCBI Accession: NM_016601

UniProt: Q9ES08