KCNN1 antibody (Intracellular)

Catalog No. ABIN7043459

The Rabbit Polyclonal anti-KCNN1 antibody has been validated for WB, IHC, IF and IC. It is suitable to detect KCNN1 in samples from Rat.

Quick Overview for KCNN1 antibody (Intracellular) (ABIN7043459)

Target: KCNN1 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 1 (KCNN1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-KCNN1 Antibody

Binding Specificity: AA 75-93, Intracellular

Purpose: A Rabbit Polyclonal Antibody to KCNN1 (KCa2.1, SK1) Channel

Specificity: Intracellular, N-terminal domain

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 19 amino acid residues identical, human - 12,Mouse - 17

Characteristics: Anti-KCNN1 (KCa2.1, SK1) Antibody (ABIN7043459, ABIN7044948 and ABIN7044949) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KCNN1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCNN1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC|Electrophysiology

Negative Control: (ABIN7236345)

Blocking Peptide: (ABIN7236345)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNN1

Target: KCNN1 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 1 (KCNN1))

Alternative Name: KCNN1

Background: SKCa1, Small conductance calcium-activated potassium channel protein 1,KCa2.1 is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.2 (SK2) and KCa2.3 (SK3). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has a similar topology to that of voltage-dependent K+ channels (KV channels), that is, six transmembrane domains and intracellular N- and C-termini. KCa2.1 channel expression is roughly confined to neuronal tissue, although there are some indications that it may be present in non-neuronal tissues as well. The sensitivity of KCa2.1 to the bee venom toxin Apamin (#STA-200) is a matter of some controversy. Expression of the human KCa2.1 in mammalian cell lines showed that most cells produce channels that can be blocked by Apamin. However, expression of KCa2.1 in Xenopus oocytes produced channels that were mostly insensitive to Apamin. Interestingly, the rat KCa2.1 is unable to form functional channels when expressed alone in either cell lines or oocytes.

Alternative names: KCNN1 (KCa2.1, SK1), SKCa1, Small conductance calcium-activated potassium channel protein 1

Gene ID: 54261

NCBI Accession: NM_002248

UniProt: P70606