KCNA1 antibody (Extracellular)

Catalog No. ABIN7043514

Product Details anti-KCNA1 Antibody

Target: KCNA1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 1 (KCNA1))

Binding Specificity: AA 193-206, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI), Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to KV1.1 (KCNA1) Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-KV1.1 (KCNA1) (extracellular) Antibody (ABIN7043514, ABIN7045058 and ABIN7045059) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry and live cell imaging applications. It has been designed to recognize KV1.1 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KDDKDFTGTIHRID, corresponding to amino acid residues 193-206 of rat KV1.1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236476)

Blocking Peptide: (ABIN7236476)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNA1

Target: KCNA1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 1 (KCNA1))

Alternative Name: KCNA1 (KCNA1 Products)

Background: Potassium voltage-gated channel subfamily A member 1,KV1.1 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.1 was the first mammalian KV channel to be cloned from mouse brain.1 Eight Shaker-related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function. The structure of KV1.1 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2The channel is expressed in neurons and cardiac and skeletal muscle tissue as well as in the retina and pancreas.2 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle. Mutations in the coding of KV1.1 gene were discovered in Episodic Ataxia patients.3KV1.1 channels are sensitive to low doses of TEA (0.3 mM) and 4-AP (0.29 mM), the "classical" non-selective potassium channel blockers.Several venomous toxins from snakes, scorpions and sea anemones are potent blockers (affecting the channels in the nanomolar range) of KV1.1 channels. Among these, the most potent and selective are α-Dendrotoxin (0.4-4 nM) and δ-Dendrotoxin (0.03-1.8 nM), Dendrotoxin-K (0.03 nM), Agitoxin-2 (0.044 nM) and Hongotoxin-1 (0.031 nM).4

Alternative names: KV1.1 (KCNA1), Potassium voltage-gated channel subfamily A member 1

Gene ID: 24520

NCBI Accession: NM_000217

UniProt: P10499