KCND2 antibody (Intracellular)

Catalog No. ABIN7043533

Product Details anti-KCND2 Antibody

Target: KCND2 (Potassium Voltage-Gated Channel, Shal-Related Subfamily, Member 2 (KCND2))

Binding Specificity: AA 454-469, Intracellular

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This KCND2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Guinea Pig Polyclonal Antibody to KV4.2 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - 15,16 amino acid residues identical

Characteristics: Guinea pig Anti-Kv4.2 Antibody is directed against an epitope of rat KV4.2 channel. Guinea pig Anti-KV4.2 Antibody (#), raised in guinea pig, can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV4.2 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-KV4.2 Antibody (ABIN7043534, ABIN7044928 and ABIN7044929)) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: BLP-PC023

Blocking Peptide: BLP-PC023

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCND2

Target: KCND2 (Potassium Voltage-Gated Channel, Shal-Related Subfamily, Member 2 (KCND2))

Alternative Name: KCND2 (KCND2 Products)

Background: Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5,KV4.2 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: KV4.1 and KV4.3.1KV4.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of KV4.1 and KV4.3.The KV4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents. For example, depolarization-activated K+ currents in rat neostriatal cholinergic interneurons are predominantly of the A-type and attributable to coexpression of KV4.2 and KV4.1 subunits.2The biophysical properties of the KV4.2 subunit can be modified by its association with auxiliary β subunits such as the KChIP family that increase KV4.2 current densities and accelerates both the inactivation and the recovery time.KV4.2 is also highly expressed in the heart where together with KV4.3 underlie the fast inactivating and recovering cardiac transient outward current Ito.3Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of KV4.2 channels. Among these the most potent and selective are Stromatoxin-1 (#STS-350), (1.2nM), Phrixotoxin-1 (#STP-700), (5 nM), Phrixotoxin-2 (#STP-710), (34 nM) and Heteropodatoxin-2 (#STH-340), (100 nM).4

Alternative names: KV4.2, Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5

Gene ID: 65180

NCBI Accession: NM_012281

UniProt: Q63881