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Nerve Growth Factor antibody (AA 84-104)

NGF Reactivity: Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043556
  • Target See all Nerve Growth Factor (NGF) Antibodies
    Nerve Growth Factor (NGF)
    Binding Specificity
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 84-104
    Reactivity
    • 70
    • 42
    • 40
    • 8
    • 7
    • 7
    • 2
    • 1
    • 1
    • 1
    Rat
    Host
    • 76
    • 17
    • 3
    • 2
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 69
    • 32
    • 1
    Polyclonal
    Conjugate
    • 65
    • 13
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Nerve Growth Factor antibody is un-conjugated
    Application
    • 61
    • 47
    • 32
    • 19
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purpose
    A Rabbit Polyclonal Antibody to proNGF
    Specificity
    Pro-domain of the NGF protein
    Cross-Reactivity
    Human, Mouse, Rat
    Cross-Reactivity (Details)
    The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.
    Predicted Reactivity
    Mouse - identical, human - 16,21 amino acid residues identical
    Characteristics
    Anti-proNGF Antibody is directed against rat proNGF. Anti-proNGF Antibody (ABIN7043556, ABIN7044751 and ABIN7044752)) can be used in western blot and immunohistochemical applications. The antibody specifically reacts with proNGF. It has been designed to recognize proNGF from mouse, rat and human samples. \nRelated products for neutralizing proNGF activity: \nMouse NGF/proNGF Neutralizing Antibody (#) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)

    Isotype
    IgG
    Top Product
    Discover our top product NGF Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Comment

    Cited Application: IP|IHC

    Negative Control: BLP-NT005

    Blocking Peptide: BLP-NT005

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    Nerve Growth Factor (NGF)
    Alternative Name
    NGF (NGF Products)
    Background
    Nerve growth factor precursor,Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins.It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8

    Alternative names: proNGF, Nerve growth factor precursor
    Gene ID
    310738
    NCBI Accession
    NM_002506
    UniProt
    P25427
    Pathways
    Regulation of Cell Size
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